Protein oxidation and lipid peroxidation after renal ischemia-reperfusion injury

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dc.authoridYAGMURCA, MURAT/0000-0001-9774-8151
dc.authoridIrmak, M. Kemal/0000-0002-1903-8303
dc.authorwosidYAĞMURCA, Murat/AAH-4496-2019
dc.authorwosidYAGMURCA, MURAT/A-1851-2018
dc.authorwosidErdogan, Hasan/AFN-9249-2022
dc.authorwosidFadillioglu, Ersin/K-3817-2019
dc.authorwosidIrmak, M. Kemal/H-6667-2014
dc.contributor.authorErdogan, H
dc.contributor.authorFadillioglu, E
dc.contributor.authorYagmurca, M
dc.contributor.authorUçar, M
dc.contributor.authorIrmak, MK
dc.date.accessioned2024-08-04T20:15:15Z
dc.date.available2024-08-04T20:15:15Z
dc.date.issued2006
dc.departmentİnönü Üniversitesien_US
dc.description29th National Congress of Physiology -- SEP 01-05, 2003 -- Ankara, TURKEYen_US
dc.description.abstractOxygen radicals have roles in the renal ischemia-reperfusion (IR) injury usually encountered in several conditions such as renal transplantation. The aim of this study was to investigate the effects of erdosteine and N-acetylcysteine (NAC) on the oxidant/antioxidant status and microscopy of renal tissues after IR injury. Male Sprague-Dawley rats were randomly assigned to four groups: control untreated rats, IR (30 min ischemia and 120 min reperfusion), IR + NAC (i.p.; 180 mg/kg) and IR + erdosteine (oral; 50 mg/kg/day for 2 days before experiments) groups. After unilateral renal IR, the right kidney was rapidly excised and sectioned vertically into two pieces for microscopic examination and biochemical analysis. Erdosteine and NAC treatment did not cause any significant change in the activity of superoxide dismutase (SOD) in comparison with the IR group, even if the SOD activity increased in IR groups than in the control group. Catalase (CAT) activity was decreased in the IR group in comparison with control and IR + erdosteine groups (P < 0.05), whereas it was higher in the IR + erdosteine group than in the IR + NAC group (P < 0.05). Xanthine oxidase (XO) activity was higher in all the IR-performed groups than in the control group (P < 0.05). Thiobarbituric acid-reactive substances (TBARS) level and protein carbonyl (PC) content were increased after IR injury (P < 0.05). Erdosteine or NAC treatments ameliorated these increased TBARS and PC contents in comparison with the IR group (P < 0.05). Light microscopy of the IR group showed tubular dilatation, tubular necrosis and vacuole formation in epithelial cells. Erdosteine but not NAC apparently reduced the renal tissue damage. The pathological damage score after IR was significantly reduced after erdosteine treatment (P < 0.05), but not after NAC treatment. In conclusion, renal IR resulted in oxidative damage as seen in biochemical lipid peroxidation and protein oxidation results with aggravated tubular necrosis. Erdosteine and NAC treatments improved the biochemical results of IR injury. However, on microscopic evaulations, animals receiving erdosteine showed a great reduction in renal damage when compared with the NAC group.en_US
dc.identifier.doi10.1007/s00240-005-0031-3
dc.identifier.endpage46en_US
dc.identifier.issn0300-5623
dc.identifier.issn1434-0879
dc.identifier.issue1en_US
dc.identifier.pmid16429300en_US
dc.identifier.scopus2-s2.0-32544441524en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage41en_US
dc.identifier.urihttps://doi.org/10.1007/s00240-005-0031-3
dc.identifier.urihttps://hdl.handle.net/11616/94270
dc.identifier.volume34en_US
dc.identifier.wosWOS:000235271700007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofUrological Researchen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectrenalen_US
dc.subjectischemia-reperfusionen_US
dc.subjecterdosteineen_US
dc.subjectN-acetylcysteineen_US
dc.subjectlight microscopyen_US
dc.subjectprotein oxidationen_US
dc.titleProtein oxidation and lipid peroxidation after renal ischemia-reperfusion injuryen_US
dc.typeConference Objecten_US

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