Cytotoxic and Pro-Apoptotic Effects of Cold-Pressed Hemp Seed Oil on Lung Cells: Cannabinoid and Fatty Acid Profiling via LC-MS/MS and GC-FID

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dc.contributor.authorYaman, Zeynep
dc.contributor.authorAydemir, Songul
dc.contributor.authorUgur, Yilmaz
dc.contributor.authorDemirtas, Sezin
dc.contributor.authorSenturk, Harun
dc.contributor.authorSalva, Emine
dc.contributor.authorErdogan, Selim
dc.date.accessioned2026-04-04T13:37:36Z
dc.date.available2026-04-04T13:37:36Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractThis study aimed to evaluate the cytotoxic and pro-apoptotic effects of cold-pressed hemp seed oil on human lung carcinoma (A549) and normal bronchial epithelial (BEAS-2B) cells. Cell viability was assessed using MTS assay across a concentration range of 1-50 mu g/mL, and a dose-dependent decline was observed in both cell types. At the highest concentration (50 mu g/mL), cell viability decreased to 27% in A549 and 22% in BEAS-2B cells, indicating limited selectivity. Flow cytometry analysis showed that treatment with 25 mu g/mL hemp seed oil resulted in a total late apoptotic/necrotic cell population of 67.13% in A549 cells, with minimal early apoptosis observed. LC-MS/MS analysis confirmed the presence of cannabidiol (CBD, 24.55 mu g/mL) and cannabinol (CBN, 11.91 mu g/mL), with excellent recovery rates (CBD: 96.10 +/- 2.38%, CBN: 98.75 +/- 1.76%) and a validated linear range of 1-1000 mu g/mL (R-2 > 0.99). GC-FID analysis identified a favorable fatty acid profile dominated by linoleic acid (55.7%), alpha-linolenic acid (18.19%), and oleic acid (14.12%), which may contribute synergistically to the observed bioactivity. While these in vitro findings suggest that hemp seed oil may possess anticancer activity, further in vivo studies are warranted to clarify its mechanisms of action, bioavailability, and therapeutic applicability.
dc.identifier.doi10.1002/slct.202504822
dc.identifier.issn2365-6549
dc.identifier.issue46
dc.identifier.orcid0000-0002-9040-4249
dc.identifier.scopus2-s2.0-105024088603
dc.identifier.scopusqualityN/A
dc.identifier.urihttps://doi.org/10.1002/slct.202504822
dc.identifier.urihttps://hdl.handle.net/11616/109934
dc.identifier.volume10
dc.identifier.wosWOS:001633792500001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofChemistryselect
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectcannabinoids
dc.subjectfatty acids
dc.subjecthemp seed oil
dc.subjectLC-MS/MS
dc.subjectlung cancer
dc.titleCytotoxic and Pro-Apoptotic Effects of Cold-Pressed Hemp Seed Oil on Lung Cells: Cannabinoid and Fatty Acid Profiling via LC-MS/MS and GC-FID
dc.typeArticle

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