Adrenomedullin ve resveratrol verilen sıçanların kahverengi ve beyaz yağ dokularında anjiogenik faktörler ve sirtuin gen ifadesinin obezite olgusunda karşılaştırılması
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Dosyalar
Tarih
2018
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/embargoedAccess
Özet
Obezite, genetik ve çevresel faktörlerin etkileşimine bağlı olarak ortaya çıkan kompleks, kronik bir hastalıktır. Adipoz dokuda olduğu gibi, pek çok anjiogenik faktör dokuların beslenmesinde yeni damarın meydana gelmesini sağlar. Adrenomedullin (AdM) ise anjiogenik özelliklere sahip bir peptiddir. Resveratrol Sirtuin 1 (SIRT1) geninin aktive edilmesinde etkili bir moleküldür. SIRT1 geni enerji metabolizmasını düzenlemede rol oynar. Obez sıçanlarda resveratrolün SIRT1 gen ifadesi üzerine etkileri, kahverengi ve beyaz yağ dokularındaki önemi nedeniyle vasküler endotelyal büyüme faktörü (VEGF)'ndeki değişimler ve AdM'in etkileri araştırıldı. Sıçanlar 8 gruba ayrıldı. Obez gruplar %60 enerji olarak yağ içeriğine sahip yüksek yağ diyetiyle 3 ay boyunca beslendi. Obezite sağlandıktan sonra AdM ve resveratrol uygulama gruplarına gün aşırı bir kez, 4 hafta süreyle 2.5 nmol/kg AdM ve 10 mg/kg resveratrol intraperitonal (i.p.) olarak uygulandı. Kahverengi ve beyaz yağ dokusundaki AdM, SIRT1 ve VEGF mRNA seviyeleri semi-kantitatif PZR ile; protein seviyeleri ise Western Blot yöntemi ile saptandı. Beyaz ve kahverengi yağ dokusu uygulamalara farklı yanıtlar vermiştir. Ayrıca obez ve kontrol grupları AdM ve resveratrol uygulamalarına farklı yanıtlar vermiştir. Beyaz adipoz dokuda SIRT1 ifadesi resveratrolle artmıştır. Kahverengi yağ dokusunda AdM'in tek başına ifadeyi artırma üzerine etkisi yoktur, fakat resveratrolle uygulandığında kontrol gruplarında ifade daha artmıştır. AdM ile resveratrol uygulaması iki molekülün ayrı ayrı uygulanmasından tamamen farklı yanıtlar ortaya çıkarmıştır. Sonuçlar AdM'in SIRT1 protein seviyelerini arttırabileceğini ve resveratrolün anjiogenezde rolü olabileceğini göstermektedir.
Obesity is a complex, chronic disease which arises according to the interaction between genetic and environmental factors. Many angiogenic factors provide formation of new vessels for the nourishment of tissues as in adipose tissue. Adrenomedullin (AdM) is also a peptide that has angiogenic features. Resveratrol is a molecule which is effective in the activation of sirtuin 1 (SIRT1) gene. SIRT1 gene has a regulatory effect on energy metabolism. The effects of resveratrol over SIRT1 gene expression, the variations of vascular endothelial growth factor (VEGF) due to their importance in development of brown and white adipose tissue, and effects of AdM were investigated. Rats were divided into 8 groups. Obese groups were fed with high fat diet which has 60% fat content as energy for 3 months. After providing obesity, 2.5 nmol/kg AdM and 10 mg/kg resveratrol were treated to experimet groups intraperitonally (i.p.) every other day for 4 weeks. AdM, SIRT1 and VEGF mRNA levels in brown and white adipose tissues were detected with semi-quantitative PCR; protein levels were detected with Western Blotting. White and brown adipose tissue groups have responsed differently to the treatments. Furthermore, obese and control groups have resposed differently to AdM and resveratrol treatments. SIRT1 expression has increased with resveratrol in white adipose tissue. AdM has no effect alone over the increase of expression, but the the expression has increased when treated with resveratrol. The treatment of AdM with resveratrol has revealed completely diverse responses than the treatment of these two molecules apart. The results have showed that AdM could have ability to increase SIRT1 protein levels, and resveratrol would have a role in angiogenesis.
Obesity is a complex, chronic disease which arises according to the interaction between genetic and environmental factors. Many angiogenic factors provide formation of new vessels for the nourishment of tissues as in adipose tissue. Adrenomedullin (AdM) is also a peptide that has angiogenic features. Resveratrol is a molecule which is effective in the activation of sirtuin 1 (SIRT1) gene. SIRT1 gene has a regulatory effect on energy metabolism. The effects of resveratrol over SIRT1 gene expression, the variations of vascular endothelial growth factor (VEGF) due to their importance in development of brown and white adipose tissue, and effects of AdM were investigated. Rats were divided into 8 groups. Obese groups were fed with high fat diet which has 60% fat content as energy for 3 months. After providing obesity, 2.5 nmol/kg AdM and 10 mg/kg resveratrol were treated to experimet groups intraperitonally (i.p.) every other day for 4 weeks. AdM, SIRT1 and VEGF mRNA levels in brown and white adipose tissues were detected with semi-quantitative PCR; protein levels were detected with Western Blotting. White and brown adipose tissue groups have responsed differently to the treatments. Furthermore, obese and control groups have resposed differently to AdM and resveratrol treatments. SIRT1 expression has increased with resveratrol in white adipose tissue. AdM has no effect alone over the increase of expression, but the the expression has increased when treated with resveratrol. The treatment of AdM with resveratrol has revealed completely diverse responses than the treatment of these two molecules apart. The results have showed that AdM could have ability to increase SIRT1 protein levels, and resveratrol would have a role in angiogenesis.
Açıklama
Anahtar Kelimeler
Fizyoloji, Physiology, Genetik, Genetics
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
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Künye
Culum, Ayşe A. Ş. (2018). Adrenomedullin ve resveratrol verilen sıçanların kahverengi ve beyaz yağ dokularında anjiogenik faktörler ve sirtuin gen ifadesinin obezite olgusunda karşılaştırılması. Yayımlanmış Doktora lisans tezi, İnönü Üniversitesi, Malatya. 1-147 ss.
