The prevalence of fecal colonization of enterococci, the resistance of the isolates to ampicillin, vancomycin, and high-level aminoglycosides, and the clonal relationship among isolates

Basit Öğe Kaydı
dc.authoridDURMAZ, RIZA/0000-0001-6561-778X
dc.authorwosidÖzerol, İbrahim Halil/ABI-8015-2020
dc.authorwosidDURMAZ, Rıza/HJH-4918-2023
dc.contributor.authorKuzucu, C
dc.contributor.authorCizmeci, Z
dc.contributor.authorDurmaz, R
dc.contributor.authorDurmaz, E
dc.contributor.authorOzerol, IH
dc.date.accessioned2024-08-04T20:14:45Z
dc.date.available2024-08-04T20:14:45Z
dc.date.issued2005
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe gastrointestinal tract carriage of enterococci was searched in 150 hospitalized patients and 100 outpatients, and clonal relatedness of the isolates and their resistance to ampicillin, vancomycin, and high-level streptomycin and gentamicin were investigated. A stool sample or rectal swab collected from each patient was inoculated into appropriate media within an hour. Enterococcus species were identified by using conventional biochemical tests, API-20 Strep assay, and BBL crystal kit. Antibiotic susceptibility tests were performed using Kirby-Bauer disk diffusion method. A polymerase chain reaction (PCR) was used to detect vanA and vanB genes. Pulsed-field gel electrophoresis (PFGE) and arbitrarily primed-polymerase chain reaction (AP-PCR) methods were used for molecular typing of the strains. Enterococci were isolated from 90 (60%) of the specimens collected from 150 inpatients. Of these 90 isolates, 37 (41%) had high-level gentamicin resistance, 36 (40%) had high-level streptomycin resistance, and 50 (55.6%) had ampicillin resistance. Fecal colonization was found in 30% of the outpatients. Resistances to ampicillin, high-level streptomycin, and gentamicin were 13%, 10%, and 3%, in these patients' isolates, respectively. No vancomycin-resistant enterococci were detected by both agar diffusion and PCR assays in our study. Both typing procedures were applied on 78 Enterococcus strains isolated from inpatients. AP-PCR typing showed that 30 (50.8%) of the 59 E. faecium and 5 (50%) of the 10 E. faecalis strains were clonally related. These values were found to be 12 (20.3%) and two (20%) by PFGE, respectively. The typing procedures did not find any clustered strains in the six E. durans and three E. avium isolates. Neither PFGE nor AP-PCR result was significantly different among the sensitive and resistant strains. Our results indicate that the high prevalence of colonization with ampicillin and high-level aminoglycoside-resistant enterococci is an important problem in our medical center. The high clonal diversity among the isolates indicates limited spread of antibiotic-resistant strains between patients.en_US
dc.identifier.doi10.1089/mdr.2005.11.159
dc.identifier.endpage164en_US
dc.identifier.issn1076-6294
dc.identifier.issn1931-8448
dc.identifier.issue2en_US
dc.identifier.pmid15910231en_US
dc.identifier.scopus2-s2.0-18944394015en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage159en_US
dc.identifier.urihttps://doi.org/10.1089/mdr.2005.11.159
dc.identifier.urihttps://hdl.handle.net/11616/93946
dc.identifier.volume11en_US
dc.identifier.wosWOS:000229308400012en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofMicrobial Drug Resistanceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectField Gel-Electrophoresisen_US
dc.subjectUnited-Statesen_US
dc.subjectHospitalsen_US
dc.subjectCarriageen_US
dc.subjectFaeciumen_US
dc.subjectSpreaden_US
dc.subjectSurveillanceen_US
dc.subjectInfectionen_US
dc.titleThe prevalence of fecal colonization of enterococci, the resistance of the isolates to ampicillin, vancomycin, and high-level aminoglycosides, and the clonal relationship among isolatesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu