Erkek sıçanlara intraserebroventriküler asprosin uygulamasının üreme hormonları üzerine etkileri
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Dosyalar
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Asprosin beyaz yağ dokudan salgılanan ve hepatik glikoz salınımını arttıran protein yapılı bir hormondur. Asprosinin beyin ve testis dokusunda lokalize olduğu, bununla birlikte asprosin uygulamasının spermlerin ilerleyici hareketini arttırdığı bilinmektedir. Bu çalışma, merkezi asprosin infüzyonunun hipotalamus hipofiz testiküler aksında görev alan hormonlar ile sperm hücreleri üzerindeki etkilerini araştırmak amacıyla yapıldı. Materyal ve Metot: Çalışmada 40 adet Spraque Dawley ırkı erkek sıçan kullanıldı. Sıçanlar kontrol, sham, 34 ve 68 nM asprosin olmak üzere dört gruba ayrıldı (n=10). Kontrol grubuna herhangi bir uygulama yapılmazken sham ve deney gruplarındaki sıçanların lateral ventriküllerine beyin infüzyon kiti implant edildi ve 14 gün süresince infüzyon (Sham grubu için yapay beyin omurilik sıvısı, uygulama grupları için 34 ve 68 nM asprosin) gerçekleştirildi. Deney sonunda sıçanların hipotalamus, kan ve epididimis dokuları toplandı. Hipotalamus dokusunda RT-PCR ve Western Blot yöntemleriyle GnRH mRNA ve doku protein düzeyleri belirlendi. Kan örneklerinden ELISA yöntemi kullanılarak serum LH, FSH ve testosteron seviyeleri, epididimis dokusunda ise sperm hücreleri incelendi. Bulgular: Asprosin uygulanan grupların GnRH mRNA ve protein ifadeleri kontrol ve sham gruplarına kıyasla yüksekti (p<0.05). Asprosin infüzyonunun serum FSH, LH ve testosteron seviyelerini de arttırdığı belirlendi (p<0.05). Ayrıca asprosin uygulanan gruplarda sperm yoğunluğunun, hareketliliğinin ve ileri hareketinin arttığı görüldü (p<0.05). Sonuç: Bu çalışma merkezi olarak uygulanan asprosinin hipotalamus hipofiz testiküler aksta görev alan hormonlar ve epididimis dokusu üzerinde aktive edici özelliğe sahip olabildiğini ortaya koymaktadır. Elde edilen sonuçlar asprosinin infertilite tedavisinde ele alınması gereken önemli bir protein/biyobelirteç olduğunu düşündürmektedir. Anahtar Kelimeler: Asprosin, FSH, GnRH, LH, Testosteron, Sperm.
Aim: Asprosin is a protein hormone secreted from white adipose tissue and increases hepatic glucose release. Known that asprosin is localized in brain and testicular tissue, however, administration of asprosin increases progressive movement of sperms. This study was carried out to investigate effects of central asprosine infusion on hormones and sperm cells in hypothalamus pituitary testicular axis. Materials and Methods: Forty male Sprague Dawley rats were used in study. Rats were divided into four groups control, sham, 34 and 68nM asprosin (n=10). While application was't made to control group, brain infusion kit was implanted into lateral ventricles of rats in sham and experimental groups infusion (artificial cerebrospinal fluid for sham, 34 and 68nM asprosine for administration groups) was performed 14 days. At end of experiment, hypothalamus, blood and epididymis tissues of rats were collected. GnRH mRNA and protein levels were determined in hypothalamus tissue by RT-PCR and Western Blot methods. Serum LH, FSH and testosterone levels were analyzed from blood samples using ELISA method, and sperm cells in epididymis tissue. Results: GnRH mRNA and protein expressions of asprosin treated groups were higher than control and sham groups (p<0.05). It was determined that asprosin infusion also increased serum FSH, LH and testosterone levels (p<0.05). In addition, sperm density, motility and forward movement were found to be increased in asprosin applied groups (p<0.05). Conclusion: This study demonstrates that centrally administered asprosine can activate hormones in hypothalamus pituitary testicular axis and epididymis tissue. Results obtained suggest that asprosin is important protein/biomarker that should be considered in treatment of infertility. Keywords: Asprosin, FSH, GnRH, Infertility, LH, Testosterone.
Aim: Asprosin is a protein hormone secreted from white adipose tissue and increases hepatic glucose release. Known that asprosin is localized in brain and testicular tissue, however, administration of asprosin increases progressive movement of sperms. This study was carried out to investigate effects of central asprosine infusion on hormones and sperm cells in hypothalamus pituitary testicular axis. Materials and Methods: Forty male Sprague Dawley rats were used in study. Rats were divided into four groups control, sham, 34 and 68nM asprosin (n=10). While application was't made to control group, brain infusion kit was implanted into lateral ventricles of rats in sham and experimental groups infusion (artificial cerebrospinal fluid for sham, 34 and 68nM asprosine for administration groups) was performed 14 days. At end of experiment, hypothalamus, blood and epididymis tissues of rats were collected. GnRH mRNA and protein levels were determined in hypothalamus tissue by RT-PCR and Western Blot methods. Serum LH, FSH and testosterone levels were analyzed from blood samples using ELISA method, and sperm cells in epididymis tissue. Results: GnRH mRNA and protein expressions of asprosin treated groups were higher than control and sham groups (p<0.05). It was determined that asprosin infusion also increased serum FSH, LH and testosterone levels (p<0.05). In addition, sperm density, motility and forward movement were found to be increased in asprosin applied groups (p<0.05). Conclusion: This study demonstrates that centrally administered asprosine can activate hormones in hypothalamus pituitary testicular axis and epididymis tissue. Results obtained suggest that asprosin is important protein/biomarker that should be considered in treatment of infertility. Keywords: Asprosin, FSH, GnRH, Infertility, LH, Testosterone.
Açıklama
Anahtar Kelimeler
Fizyoloji, Physiology
Kaynak
WoS Q Değeri
Scopus Q Değeri
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Künye
Keskin, T. (2021). Erkek sıçanlara intraserebroventriküler asprosin uygulamasının üreme hormonları üzerine etkileri. Yayınlanmış Yüksek Lisans Tezi, İnönü Üniversitesi.
