Evaluation of diagnostic performance of new antigen-based enzyme immune assay for diagnosis of Hepatitis C virus (HCV) infections
Küçük Resim Yok
Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Journals
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Laboratory diagnosis of Hepatitis C virus (HCV) is sometimes problematic. A novel immune enzyme assay (HCV-Core Antigen, Abbott Laboratories/Germany) has been recently licensed for detection of HCV antigen in human serum. In this study, we aimed to evaluate diagnostic performance of this new test in comparison with the HCV-RNA quantification and anti-HCV antibody (anti-HCV-Ab) analyses. A total of 152 serum samples of which 112 anti-HCV-Ab positive (range 1.08 to 386.54 s/co) and 40 anti-HCV-Ab negative (<1 s/co) were analyzed with HCV-RNA and HCV-Ag tests. According to HCV-RNA detection, sensitivity and specificity of HCV-Ag test was measured as 96.9 and 100%, respectively and of anti-HCV-Ab were measured as 100 and 60%, orderly. An excellent positive predictive value for HCV-Ag test was detected as 100%, whereas 28.5% for anti-HCV-Ab test. Pearson correlation analysis showed that there was a statistically significant and strong relationship (p < 0.001, R: 0.773) between HCV-Ag and HCV-RNA quantification analysis. The correlation between the two tests showed an exponential trend (R-2: 0.949). These results suggest that HCV-Ag test may be a reliable assay for HCV antigen detection, which is also well-correlated with serum viral load. However, large studies, including different HCV genotypes and with extreme viral quantity, are required to assess analytic potency of this novel kit.
Açıklama
Anahtar Kelimeler
Hepatitis C, diagnosis, ELISA, HCV core antigen, HCV-RNA
Kaynak
African Journal of Microbiology Research
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
6
Sayı
4
