Evaluation of diagnostic performance of new antigen-based enzyme immune assay for diagnosis of Hepatitis C virus (HCV) infections

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dc.authoridözer, ali/0000-0002-7144-4915;
dc.authorwosidözer, ali/ABI-2209-2020
dc.authorwosidYAKUPOGULLARI, YUSUF/F-3966-2011
dc.contributor.authorKooglu, Mehmet
dc.contributor.authorAk, Sibel
dc.contributor.authorAk, Muharrem
dc.contributor.authorYakupogullari, Yusuf
dc.contributor.authorOzer, Ali
dc.date.accessioned2024-08-04T20:58:44Z
dc.date.available2024-08-04T20:58:44Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.description.abstractLaboratory diagnosis of Hepatitis C virus (HCV) is sometimes problematic. A novel immune enzyme assay (HCV-Core Antigen, Abbott Laboratories/Germany) has been recently licensed for detection of HCV antigen in human serum. In this study, we aimed to evaluate diagnostic performance of this new test in comparison with the HCV-RNA quantification and anti-HCV antibody (anti-HCV-Ab) analyses. A total of 152 serum samples of which 112 anti-HCV-Ab positive (range 1.08 to 386.54 s/co) and 40 anti-HCV-Ab negative (<1 s/co) were analyzed with HCV-RNA and HCV-Ag tests. According to HCV-RNA detection, sensitivity and specificity of HCV-Ag test was measured as 96.9 and 100%, respectively and of anti-HCV-Ab were measured as 100 and 60%, orderly. An excellent positive predictive value for HCV-Ag test was detected as 100%, whereas 28.5% for anti-HCV-Ab test. Pearson correlation analysis showed that there was a statistically significant and strong relationship (p < 0.001, R: 0.773) between HCV-Ag and HCV-RNA quantification analysis. The correlation between the two tests showed an exponential trend (R-2: 0.949). These results suggest that HCV-Ag test may be a reliable assay for HCV antigen detection, which is also well-correlated with serum viral load. However, large studies, including different HCV genotypes and with extreme viral quantity, are required to assess analytic potency of this novel kit.en_US
dc.identifier.doi10.5897/AJMR11.1130
dc.identifier.endpage812en_US
dc.identifier.issn1996-0808
dc.identifier.issue4en_US
dc.identifier.startpage809en_US
dc.identifier.urihttps://doi.org/10.5897/AJMR11.1130
dc.identifier.urihttps://hdl.handle.net/11616/103107
dc.identifier.volume6en_US
dc.identifier.wosWOS:000306480900015en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.relation.ispartofAfrican Journal of Microbiology Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHepatitis Cen_US
dc.subjectdiagnosisen_US
dc.subjectELISAen_US
dc.subjectHCV core antigenen_US
dc.subjectHCV-RNAen_US
dc.titleEvaluation of diagnostic performance of new antigen-based enzyme immune assay for diagnosis of Hepatitis C virus (HCV) infectionsen_US
dc.typeArticleen_US

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