Metabolomics approach using HR-MAS NMR spectroscopy for the assessment of metabolic profiles of uterine fibroids

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dc.contributor.authorDuz, Senem Arda
dc.contributor.authorMumcu, Akin
dc.contributor.authorDogan, Berat
dc.contributor.authorSaridogan, Erdinc
dc.contributor.authorTuncay, Gorkem
dc.contributor.authorOnat, Taylan
dc.contributor.authorKaraer, Abdullah
dc.date.accessioned2026-04-04T13:35:16Z
dc.date.available2026-04-04T13:35:16Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractThe aim of this study is to determine dysregulated metabolites and metabolic pathways in uterine fibroids and in the myometrial tissue from which uterine fibroids are derived. Fifteen (15) patients underwent hysterectomy because of uterine fibroids and 14 controls were included in this study. 1H HR-MAS NMR spectroscopy data were obtained from uterine fibroid tissue, the adjacent healthy myometrial tissue from cases, and myometrial tissue from controls. PCA and PLS-DA score plots from multivariate statistical analysis of pre-processed spectral data demonstrated a distinction between cases and control groups. The levels of lactate, alanine, glutamate, glutamine, methionine, acetone, isocitrate, choline, glycerophosphocholine, phosphocholine, o-phosphoethanol-amine, taurine, myo-inositol, p-methylhistidine, phenylacetate, ascorbate, glucose, and methylhistidine were significantly higher in uterine fibroid tissue compared to the neighboring healthy myometrial tissue. Additionally, when adjacent healthy myometrial tissue was compared to control myometrial tissue, significantly lower levels of valine, leucine, isoleucine, ethanol, arginine, N-acetyl tyrosine, acetone, p-methylhistidine, glucose, phenylacetate, myo-inositol, and alpha-glucose were observed. The study provides a foundational framework by revealing the metabolomic heterogeneity of uterine fibroids. Strategies should be developed to target the metabolic alterations that contribute to the growth of these common tumors.
dc.description.sponsorshipInonu University Scientific Research Projects Coordination Unit [TCD-2021-2356]
dc.description.sponsorshipThis study was supported by the Inonu University Scientific Research Projects Coordination Unit, Project Number: TCD-2021-2356.
dc.identifier.doi10.1016/j.ab.2025.115885
dc.identifier.issn0003-2697
dc.identifier.issn1096-0309
dc.identifier.orcid0000-0003-4810-1970
dc.identifier.pmid40311774
dc.identifier.scopus2-s2.0-105004547886
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.ab.2025.115885
dc.identifier.urihttps://hdl.handle.net/11616/109722
dc.identifier.volume704
dc.identifier.wosWOS:001491137000001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.ispartofAnalytical Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250329
dc.subjectUterine fibroids
dc.subjectMyometrium
dc.subjectNMR spectroscopy
dc.subjectMetabolomics
dc.titleMetabolomics approach using HR-MAS NMR spectroscopy for the assessment of metabolic profiles of uterine fibroids
dc.typeArticle

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