In Vitro PDGF-B Gene Silencing Studies and In Vivo Delivery of siRNA to the Rat Kidney Using Chitosan/siRNA Nanoplexes

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dc.authoridAlan, Saadet/0000-0003-2329-151X
dc.authoridSalva, Emine/0000-0002-1159-5850
dc.authoridŞALVA, EMINE/0000-0002-1159-5850
dc.authorwosidAlan, Saadet/ABH-4282-2020
dc.authorwosidSalva, Emine/ABI-2766-2020
dc.authorwosidŞALVA, EMINE/CAH-3062-2022
dc.contributor.authorSalva, Emine
dc.contributor.authorOzbas Turan, Suna
dc.contributor.authorAlan, Saadet
dc.contributor.authorAkbuga, Julide
dc.date.accessioned2024-08-04T20:42:41Z
dc.date.available2024-08-04T20:42:41Z
dc.date.issued2016
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe targeting of specific genes responsible from onset and progression of kidney diseases offer a new therapeutic strategy in the field of renal gene therapy. The altered expression of platelet derived growth factor (PDGF) is an important marker of renal diseases. In this study, we investigated in vitro gene silencing efficiency of chitosan nanoplexes containing PDGF-B and PDGFR-beta targeted siRNAs in the kidney cell lines including HEK-293 and MDCK and delivery to the kidney as an in vivo delivery system. As a result, PDGF-B expression was significantly inhibited by co-delivery of chitosan/siPDGF-B+siPDGFR-beta nanoplexes prepared using in the different weight ratios (10/1, 20/1 and 50/1). When 20/1 and 50/1 weight ratios of chitosan nanoplexes were i.v. injected to rats, chitosan/FITC-siPDGFB nanoplexes were reached to kidney tissue at 4 h after intravenous injection. These results suggest that delivery of siRNA using chitosan nanoplexes may be effective for the therapy of kidney diseases.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [113S202]en_US
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK, 113S202)en_US
dc.identifier.doi10.12991/mpj.20162082721
dc.identifier.endpage268en_US
dc.identifier.issn1309-0801
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-84988638054en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage263en_US
dc.identifier.urihttps://doi.org/10.12991/mpj.20162082721
dc.identifier.urihttps://hdl.handle.net/11616/97512
dc.identifier.volume20en_US
dc.identifier.wosWOS:000391173600005en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMarmara Univ, Fac Medicineen_US
dc.relation.ispartofMarmara Pharmaceutical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectsiRNAen_US
dc.subjectPDGF-Ben_US
dc.subjectPDGFR-betaen_US
dc.subjectchitosanen_US
dc.subjectnanoplexesen_US
dc.subjectkidneyen_US
dc.titleIn Vitro PDGF-B Gene Silencing Studies and In Vivo Delivery of siRNA to the Rat Kidney Using Chitosan/siRNA Nanoplexesen_US
dc.typeArticleen_US

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