Few genes are associated with the capability of pancreatic ductal adenocarcinoma cells to grow in the liver of nude rats

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dc.authoridKleeff, Jorg/0000-0003-3432-6669
dc.authorwosidKleeff, Jorg/B-2124-2009
dc.contributor.authorEyol, Erguel
dc.contributor.authorMurtaga, Ahmed
dc.contributor.authorZhivkova-Galunska, Maria
dc.contributor.authorGeorges, Rania
dc.contributor.authorZepp, Michael
dc.contributor.authorDjandji, Dominik
dc.contributor.authorKleeff, Joerg
dc.date.accessioned2024-08-04T20:36:11Z
dc.date.available2024-08-04T20:36:11Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.description.abstractOwing to aggressiveness and chemoresistance, pancreatic ductal adenocarcinoma (PDAC) is characterised by a poor prognosis. To address this disease-specific dilemma we aimed to establish animal models, which can be used for identifying new specific tumor markers, as well as serving as tools for potential therapeutic approaches. From a panel of sixteen pancreatic cancer cell lines, two human (Suit2-007 and Suit2-013) and a rat (ASML) cell line were selected for their properties to grow in the liver of male RNU rats and mimic liver metastasis of PDAC. For better monitoring of metastatic tumor growth in vivo, all three pancreatic cancer cell lines were stably transfected with eGFP and luciferase marker genes. In addition, the mRNA expression profile of 13 human PDAC cell lines was analyzed by Bead Chip array analysis. Only 33 genes and 5 signaling pathways were identified as significantly associated with the ability of the cell lines to grow initially and/or consistently in rat liver. Only a minority of these genes (osteopontin, matrix metalloproteinase-1 and insulin-like growth factor 1) has been intensively studied and shown to be closely related to cancer progression. The function of the remaining 30 genes ranges from moderate to poorly investigated, and their function in cancer progression is still unclear. The ensuing three pancreatic cancer liver metastasis models vary in their aggressiveness and macroscopic growth. They will be used for preclinical evaluation of new therapeutic approaches aiming at the genes identified.en_US
dc.identifier.doi10.3892/or.2012.2049
dc.identifier.endpage2187en_US
dc.identifier.issn1021-335X
dc.identifier.issn1791-2431
dc.identifier.issue6en_US
dc.identifier.pmid23007550en_US
dc.identifier.scopus2-s2.0-84868132805en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2177en_US
dc.identifier.urihttps://doi.org/10.3892/or.2012.2049
dc.identifier.urihttps://hdl.handle.net/11616/95838
dc.identifier.volume28en_US
dc.identifier.wosWOS:000311984800034en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpandidos Publ Ltden_US
dc.relation.ispartofOncology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectmicroarrayen_US
dc.subjectpancreatic ductal adenocarcinoma cell linesen_US
dc.subjectliver metastasisen_US
dc.subjectanimal modelsen_US
dc.titleFew genes are associated with the capability of pancreatic ductal adenocarcinoma cells to grow in the liver of nude ratsen_US
dc.typeArticleen_US

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