Investigation of the effect of milrinone on renal damage in an experimental non-heart beating donormodel
Yükleniyor...
Dosyalar
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
TAYLOR & FRANCIS INC, 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Purpose: In our study, it was aimed to investigate the preventive effect of milrinone on renal damage in experimental controlled non-heart-beating donors (NHBDs) model. Materials and Methods: Sixteen rats randomly divided into 2 groups, 8 rats in each were used. Group 1 was control, group 2 was milrinone group. Group 1 rats received 1.25ml 0.09% NaCl intraperitoneally equivalent to the milrinone diluted volume. Group 2 rats were administered intraperitoneally with 0.5mg/kg of milrinone 2hours before cardiac arrest. After the cardiac arrest, left nephrectomy was applied to the rats. Malondialdehyde, superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) activities, Caspase-3 (apoptotic index) and histopathological evaluation were performed in the tissues. Results: In the milrinone group, the total injury score was significantly lower relative to the control group (p = 0.001). Caspase-3 staining was moderately strong in the control group but weaker in the milrinone group. Apoptotic index was significantly lower in the milrinone group compared to the control group (p = 0.001). In comparison between groups, SOD and GPx in the milrinone group was significantly higher than the control group (p = 0.008, p = 0.006). Conclusions: Milrinone has been shown to be effective in the prevention of tissue damage due to oxidative stress and inflammatory process in the renal of warm ischemia in the experimental NHBDs model and in protecting the renal. Milrinone increases antioxidant activity while reducing apoptosis. Systemic administration of milrinone prior to cardiac arrest may be beneficial. Administration of milrinone to the recipient in the perioperative period may contribute to donor function.
Açıklama
Anahtar Kelimeler
Ischemıa-Reperfusıon Injury, Tumor-Necrosıs-Factor, Kıdney-Transplantatıon, Oxıdatıve Damage, Warm Ischemıa, Lıver-Damage, Apoptosıs, Rats, Attenuatıon, Grafts
Kaynak
JOURNAL OF INVESTIGATIVE SURGERY
WoS Q Değeri
Scopus Q Değeri
Cilt
31
Sayı
5
Künye
Batcıoğlu, K. Uyumlu, BA. Karadağ, M. (2018). Investigation of the effect of milrinone on renal damage in an experimental non-heart beating donormodel. Cilt:31 Sayı:5, 402-411 ss.
