The roles of SFKs in the regulation of proinflammatory cytokines and NLRP3 in familial mediterranean fever patients

Basit Öğe Kaydı
dc.authorscopusid57203239710
dc.authorscopusid58539600400
dc.authorscopusid57196914953
dc.authorscopusid57190951817
dc.authorscopusid54386385600
dc.authorscopusid57306109600
dc.contributor.authorİçen Taşkın I.
dc.contributor.authorGürbüz S.
dc.contributor.authorKoç A.
dc.contributor.authorKocabay S.
dc.contributor.authorYolbaş S.
dc.contributor.authorKeser M.F.
dc.date.accessioned2024-08-04T20:00:44Z
dc.date.available2024-08-04T20:00:44Z
dc.date.issued2024
dc.departmentİnönü Üniversitesien_US
dc.description.abstractFamilial Mediterranean Fever (FMF) is caused by mutations in pyrin, a protein produced in innate immune cells that regulates the development of interleukin (IL)-1? by interacting with caspase-1 and other components of inflammasomes. Although overexpression of proinflammatory cytokines have been observed in FMF patients, no studies have been conducted on the role of Src family kinases (SFKs). The purpose of this study was to examine the impact of SFKs on the modulation of IL-1?, IL-6, IL-8, TNF-?, and NLRP3 inflammasome in patients with FMF. The study included 20 FMF patients and 20 controls. Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation. Protein expression levels of SFKs members were measured by western blot. The effect of lipopolysaccharide-induced (LPS) activation and PP2- induced inhibition of SFKs on NLRP3 and IL-1?, IL 6, IL-8, TNF-? were examined by western blot and flow cytometry respectively. Patients with FMF have considerably greater levels of Lck expression. In addition, patients had a substantially greater basal level of NLRP3 than the control group (*p = 0.016). Most importantly, the levels of IL-1 ? were elevated with LPS stimulation and reduced with PP2 inhibition in FMF patients. These results suggest that SFKs are effective in regulation of IL-1 ? in FMF patients. © 2024 Elsevier Ltden_US
dc.description.sponsorshipTCD-2020-1997en_US
dc.description.sponsorshipThis work was supported by Inonu University Scientific Research Projects Unit (grand number: TCD-2020-1997).en_US
dc.identifier.doi10.1016/j.cyto.2024.156615
dc.identifier.issn1043-4666
dc.identifier.pmid38640560en_US
dc.identifier.scopus2-s2.0-85190416421en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.cyto.2024.156615
dc.identifier.urihttps://hdl.handle.net/11616/90951
dc.identifier.volume179en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Pressen_US
dc.relation.ispartofCytokineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFamilial mediterranean feveren_US
dc.subjectNLRP3en_US
dc.subjectPBMCsen_US
dc.subjectProinflammatory cytokinesen_US
dc.subjectSrc family kinaseen_US
dc.titleThe roles of SFKs in the regulation of proinflammatory cytokines and NLRP3 in familial mediterranean fever patientsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu