Periodontal hastalığın patogenezinde sirkadiyen saat genlerinin incelenmesi
Küçük Resim Yok
Tarih
2025
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Sirkadiyen ritim doku ve hücrelerin fizyolojik aktivitelerini düzenleyen bir döngüdür. Sirkadiyen ritimdeki düzensizliklerin otoimmün hastalıklar ve kanser gibi çeşitli hastalıklarla ilişkili olduğu gösterilmiştir. Bu çalışmanın amacı benzer uyku döngüsüne fakat farklı periodontal hastalığa sahip bireylerin dişeti dokusunda, sirkadiyen ritim ile ilişkili yapıların gen ve protein düzeyini inceleyerek periodontal hastalığın sirkadiyen ritim üzerine olan etkilerini araştırmaktır. Materyal ve Metot: Çalışmaya sistemik olarak sağlıklı ve sigara kullanmayan, benzer uyku düzenine fakat farklı periodontal hastalığa sahip 60 birey (periodontal olarak sağlıklı [n=20; 10 erkek ve 10 kadın], gingivitis [n=20; 10 erkek ve 10 kadın] ve periodontitis [n=20; 10 erkek ve 10 kadın]) dahil edilmiştir. Katılımcılardan toplanan dişeti örneklerinde sirkadiyen ritim ile ilişkili yapılardan BMAL1, CLOCK, CRY1, CRY2, Per1, Per2, Per3, Rev-Erb-?, ROR-?'nın gen ve protein düzeyleri Western Blot ve RT-qPCR ile analiz edilmiştir. İnflamasyonla ilişkili yapılar IL-1?, IL-6, TNF-?, NF-?B, IFN-?, RANKL ve OPG gen ekspresyon düzeyleri RT-qPCR yöntemiyle analiz edilirken, IL-1? ve IL-6 protein seviyeleri ELISA ile saptanmıştır. Bulgular: Periodontitisli hastalarda dişetinde CLOCK ve Per3 gen ve protein düzeyleri anlamlı oranda yüksek; CRY1, CRY2, Per1, Per2 ve Rev-Erb-? gen ve protein düzeyleri ise düşük bulunmuştur (p<0.05). İnflamatuar yapılardan IL-1?, IL-6, TNF-?, NF-?B ve IFN-? ile CLOCK ve Per3 arasında pozitif bir korelasyon saptanırken, aynı yapılar ile CRY1, CRY2, Per1, Per2 ve Rev-Erb-? arasında ise negatif bir korelasyon saptanmıştır (p<0.05). Sonuç: Periodontitis varlığı, benzer uyku döngüsüne sahip bireylerin dişetindeki sirkadiyen saat gen ve protein ekspresyonunu değiştirerek sirkadiyen ritmi bozabilir. Sirkadiyen saat genleri periodontal hastalıklardaki inflamatuar süreçlerle etkileşimde olabilir. Anahtar Kelimeler: CRY1, İnflamasyon, Sirkadiyen Ritim, Saat Genleri, Periodontitis, Per1
Investigation of Circadian Clock Genes in the Pathogenesis of Periodontal Disease Objective: The circadian rhythm regulates the physiological activities of tissues and cells. Disruptions in the circadian rhythm have been linked to diseases such as autoimmune disorders and cancer. This study aims to investigate the effects of periodontal disease on circadian rhythm by examining the gene and protein levels of circadian rhythm-related structures in the gingival tissue of individuals with similar sleep cycles but different periodontal diseases. Materials and Methods: The study included 60 systemically healthy, non-smoking individuals with similar sleep patterns but different periodontal diseases (periodontally healthy [n=20; 10 men and 10 women], gingivitis [n=20; 10 men and 10 women], periodontitis [n=20; 10 men and 10 women]). Gene and protein levels of circadian rhythm-related structures BMAL1, CLOCK, CRY1, CRY2, Per1, Per2, Per3, Rev-Erb-?, ROR-? in gingival samples were analyzed by Western Blot and RT-qPCR. Inflammation-related markers IL-1?, IL-6, TNF-?, NF-?B, IFN-?, RANKL, and OPG were analyzed by RT-qPCR, and IL-1? and IL-6 protein levels by ELISA. Results: In periodontitis patients, CLOCK and Per3 gene and protein levels were significantly higher, while CRY1, CRY2, Per1, Per2, and Rev-Erb-? were lower (p<0.05). Inflammatory parameters IL-1?, IL-6, TNF-?, NF-?B, and IFN-? were positively correlated with CLOCK and Per3, but they negatively correlated with CRY1, CRY2, Per1, Per2 and Rev-Erb-? (p <0.05). Conclusion: Periodontitis may disrupt the circadian rhythm by altering circadian clock gene and protein expression in the gingiva. Circadian clock genes may interact with inflammatory processes in periodontal diseases. Keywords: Circadian Rhythm, Clock Genes, CRY1, Inflammation, Per1, Periodontitis
Investigation of Circadian Clock Genes in the Pathogenesis of Periodontal Disease Objective: The circadian rhythm regulates the physiological activities of tissues and cells. Disruptions in the circadian rhythm have been linked to diseases such as autoimmune disorders and cancer. This study aims to investigate the effects of periodontal disease on circadian rhythm by examining the gene and protein levels of circadian rhythm-related structures in the gingival tissue of individuals with similar sleep cycles but different periodontal diseases. Materials and Methods: The study included 60 systemically healthy, non-smoking individuals with similar sleep patterns but different periodontal diseases (periodontally healthy [n=20; 10 men and 10 women], gingivitis [n=20; 10 men and 10 women], periodontitis [n=20; 10 men and 10 women]). Gene and protein levels of circadian rhythm-related structures BMAL1, CLOCK, CRY1, CRY2, Per1, Per2, Per3, Rev-Erb-?, ROR-? in gingival samples were analyzed by Western Blot and RT-qPCR. Inflammation-related markers IL-1?, IL-6, TNF-?, NF-?B, IFN-?, RANKL, and OPG were analyzed by RT-qPCR, and IL-1? and IL-6 protein levels by ELISA. Results: In periodontitis patients, CLOCK and Per3 gene and protein levels were significantly higher, while CRY1, CRY2, Per1, Per2, and Rev-Erb-? were lower (p<0.05). Inflammatory parameters IL-1?, IL-6, TNF-?, NF-?B, and IFN-? were positively correlated with CLOCK and Per3, but they negatively correlated with CRY1, CRY2, Per1, Per2 and Rev-Erb-? (p <0.05). Conclusion: Periodontitis may disrupt the circadian rhythm by altering circadian clock gene and protein expression in the gingiva. Circadian clock genes may interact with inflammatory processes in periodontal diseases. Keywords: Circadian Rhythm, Clock Genes, CRY1, Inflammation, Per1, Periodontitis
Açıklama
Anahtar Kelimeler
Diş Hekimliği, Dentistry
