Non-Hodgkin lenfomalı hastalardan elde edilen periferik stem cell ve kemik iliği hücrelerinin natural killer aktivitesi üzerine interleukin-2’nin etkisi
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Dosyalar
Tarih
1996
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi Tıp Fakültesi Dergisi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Non-Hodgkin lenfomalı 24 hastadan elde edilen periferik stem cell (PSC) ürünlerinin natural killer (NK) hücre aktivitesi, IL-2 ile kültürden önce ve 5 gün IL-2 ile birlikte kültür yapılarak incelendi. Bu çalışmaya göre PSC ürünleri IL-2 ile stimüle edilmeden önce kemik iliği (BM) ürünlerinden daha yüksek NK celi aktivitesine sahip (p<0.001) iken normal periferik kan lökositleri (PBL)’ne eşdeğer aktivite gösterdi. Interleukin-2 aktivasyonundan sonra, PSC ve BM hücrelerinin NK hücre aktivitesi, normal donörlerden elde edilen PBL ’ye göre daha düşüktü (p<0.001). Lökositler IL-2 ile 5 gün kültür yapılınca, BM hücrelerinin NK aktivitesi hem PBL hem de PSC’den anlamlı olarak daha düşük (p<0.01) iken PBL ve PSC arasında farklılık gözlenmedi. IL-2 ile kültür öncesine göre PSC, BM ve PBL ’nin NK aktivitesi, kültürden sonra, sırasıyla 176, 14 ve 40 kat artma gösterdi (tümü için p<0.001). Bu sonuçlar, PSC transplantasyonundan önce PSC'in in vitro IL-2 ile muamele edilmesi yoluyla defektif NK-sitotoksisitesini düzeltilebileceğini göstermektedir.
The activity of natural killer (NK) cells of peripheral stem cell (PSC) products from 24 patients with non-Hodgkin's lymphoma (NHL) were examined both before and following 5 days co-incubation with IL-2. This study showed that the PSC products prior to IL-2 incubation had higher NK activity than bone marrow (BM) products (p<0.001), but with activity similar to normal peripheral blood leukocytes (PBL). Following IL-2 activation, PSC and BM cells had lower NK activity compared to PBL from normal donors (p<0.001). When leukocytes were cultured in the presence of IL-2 for 5 days, the NK activity of BM cells was significantly lower than both PBL and PSC (p<0.01), but there was no difference between PBL and PSC. The NK activities of PSC, BM and PBL were increased 176, 14 and 40 folds compared to cells prior to IL-2 culture (all of these p<0.001). ^s these results show, ex vivo threat of PSC before PSC transplantation is capable of to increase defective NK-cytotoxicity.
The activity of natural killer (NK) cells of peripheral stem cell (PSC) products from 24 patients with non-Hodgkin's lymphoma (NHL) were examined both before and following 5 days co-incubation with IL-2. This study showed that the PSC products prior to IL-2 incubation had higher NK activity than bone marrow (BM) products (p<0.001), but with activity similar to normal peripheral blood leukocytes (PBL). Following IL-2 activation, PSC and BM cells had lower NK activity compared to PBL from normal donors (p<0.001). When leukocytes were cultured in the presence of IL-2 for 5 days, the NK activity of BM cells was significantly lower than both PBL and PSC (p<0.01), but there was no difference between PBL and PSC. The NK activities of PSC, BM and PBL were increased 176, 14 and 40 folds compared to cells prior to IL-2 culture (all of these p<0.001). ^s these results show, ex vivo threat of PSC before PSC transplantation is capable of to increase defective NK-cytotoxicity.
Açıklama
[Turgut Özal Tıp Merkezi Dergisi 1996;3(3):177-182]
Anahtar Kelimeler
Non-Hodgkin lenfoma, IL-2, NK sitotoksisitesi, Kemik iliği hücreleri, Periferik stem cell
Kaynak
Turgut Özal Tıp Merkezi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
3
Sayı
3
Künye
Özerol, İ. Halil.,Şenol, M.,Ageitos, A.,Talmadge, James E.,(1996). Non-Hodgkin lenfomalı hastalardan elde edilen periferik stem cell ve kemik iliği hücrelerinin natural killer aktivitesi üzerine interleukin-2’nin etkisi.Turgut Özal Tıp Merkezi Dergisi, 3 (3).177-182 ss.
