Use of microfluidic sperm extraction chips as an alternative method in patients with recurrent in vitro fertilisation failure

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dc.authoridYuksel, Sengul/0000-0002-7190-431X
dc.authorwosidyildiz, Koray/AAD-2855-2019
dc.authorwosidYuksel, Sengul/ABI-2182-2020
dc.contributor.authorYildiz, Koray
dc.contributor.authorYuksel, Sengul
dc.date.accessioned2024-08-04T20:45:59Z
dc.date.available2024-08-04T20:45:59Z
dc.date.issued2019
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPurpose It is known that sperm preparation techniques in in vitro fertilisation (IVF) are intended to select the best-quality sperm. The aim of this study is to compare sperm the density gradient method and microfluidic chip (Fertile Plus) method in infertile patients by analysing fertilisation rates, pregnancy rates, and sperm morphology and DNA fragmentation rates posed by these two methods. Methods Using semen samples obtained from the patients, sperms were prepared with gradient (n = 312) and microfluidic chip methods (n = 116). Fertilisation and pregnancy rates were compared in the first time and in the recurrent IVF trial patients. In addition, the morphology and DNA fragmentation comparison of sperm samples were evaluated by Toluidine blue in situ chemical staining method. Results There was no statistically significant difference between fertilisation and pregnancy rates when compared with study groups in first-time IVF treatment patients. However, in recurrent IVF failure patients, there was a significant difference in fertilisation rates but no statistically significant difference was found in pregnancy rates. The microfluidic chip method significantly decreased sperm DNA fragmentation index according to density gradient method. Conclusions Microfluidic chip method may be recommended in patients with recurrent unsuccessful in vitro trials. The sperm DNA fragmentation test prior to the treatment will be helpful in selecting the appropriate sperm-washing method.en_US
dc.description.sponsorshipInonu University Research Fund [2016/147]en_US
dc.description.sponsorshipThis study was funded by Inonu University Research Fund (2016/147).en_US
dc.identifier.doi10.1007/s10815-019-01480-3
dc.identifier.endpage1429en_US
dc.identifier.issn1058-0468
dc.identifier.issn1573-7330
dc.identifier.issue7en_US
dc.identifier.pmid31093866en_US
dc.identifier.scopus2-s2.0-85066044247en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1423en_US
dc.identifier.urihttps://doi.org/10.1007/s10815-019-01480-3
dc.identifier.urihttps://hdl.handle.net/11616/98806
dc.identifier.volume36en_US
dc.identifier.wosWOS:000476540700014en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer/Plenum Publishersen_US
dc.relation.ispartofJournal of Assisted Reproduction and Geneticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectIVFen_US
dc.subjectMicrofluidic chipen_US
dc.subjectSpermen_US
dc.subjectDNA fragmentationen_US
dc.titleUse of microfluidic sperm extraction chips as an alternative method in patients with recurrent in vitro fertilisation failureen_US
dc.typeArticleen_US

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