The effects of chitosan/miR-200c nanoplexes on different stages of cancers in breast cancer cell lines

Basit Öğe Kaydı
dc.authoridŞALVA, EMINE/0000-0002-1159-5850
dc.authoridSalva, Emine/0000-0002-1159-5850
dc.authoridKaban, Kübra/0000-0001-7311-7187
dc.authorwosidŞALVA, EMINE/CAH-3062-2022
dc.authorwosidSalva, Emine/ABI-2766-2020
dc.authorwosidKaban, Kübra/JFB-1837-2023
dc.contributor.authorKaban, Kubra
dc.contributor.authorSalva, Emine
dc.contributor.authorAkbuga, Julide
dc.date.accessioned2024-08-04T20:41:48Z
dc.date.available2024-08-04T20:41:48Z
dc.date.issued2016
dc.departmentİnönü Üniversitesien_US
dc.description.abstractDysregulation of miR-200c in breast cancer has been associated with migration, epithelial mesenchymal transition (EMT), angiogenesis and metastasis of the tumor cells. Therefore, the modulation of miR-200c offers a promising therapeutic approach in breast cancer. However, the major obstacles in the usage of miRNAs in therapy are their low stability, rapid clearance, and poor cellular uptake. The development of efficient and safe delivery systems is important in effective therapy with miRNA. The purpose of this study was to investigate the therapeutic role of chitosan/miR-200c nanoplexes in angiogenesis, EMT, invasion, and apoptosis in breast cancer cell lines. We found that miR-200c levels were downregulated in various breast cancer cell lines by qRT-PCR After transfection with chitosan/miRNA nanoplexes in the appropriate size (294 nm) and zeta potential (12.3 mV), levels of miR-200c increased and reached the endogenous miR-200c levels in the MCF-7, MDA-MB-231, and MDA-MB-435 cells. While the chitosan/miR-200c nanoplexes decreased angiogenesis, invasion, EMT, and metastasis in the cells, the apoptosis levels increased by 3.1, 1.3, and 3 fold in the MCF-7, MDA-MB-231, MDA-MB-435 cell lines, respectively. Consequently, chitosan is a suitable carrier for miR-200c and formed stable nanoplexes with miR-200c. The effect of the chitosan/miRNA nanoplexes on tumor angiogenesis, EMT, invasion, metastasis, and apoptosis, changed depending on the cell-types. Therefore, during the treatment with the chitosan based miR-200c nanoplexes in breast cancers, the type of tumor cells must be considered. (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipMarmara University Scientific Research Projects Association [SAG-C-YLP-120514-0137]en_US
dc.description.sponsorshipThis study was supported by the Marmara University Scientific Research Projects Association (SAG-C-YLP-120514-0137). We would like to thank to Prof. Dr. Oguz Ozturk for kindly providing the MCF-10A cell line.en_US
dc.identifier.doi10.1016/j.ejps.2016.05.030
dc.identifier.endpage110en_US
dc.identifier.issn0928-0987
dc.identifier.issn1879-0720
dc.identifier.pmid27260087en_US
dc.identifier.scopus2-s2.0-84973569712en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage103en_US
dc.identifier.urihttps://doi.org/10.1016/j.ejps.2016.05.030
dc.identifier.urihttps://hdl.handle.net/11616/97364
dc.identifier.volume95en_US
dc.identifier.wosWOS:000390505100013en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofEuropean Journal of Pharmaceutical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectmiR-200cen_US
dc.subjectChitosanen_US
dc.subjectBreast canceren_US
dc.subjectmiRNA delivery systemen_US
dc.titleThe effects of chitosan/miR-200c nanoplexes on different stages of cancers in breast cancer cell linesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu