Effect of vgb gene on microbial chondroitin sulfate production in recombinant Escherichia coli pETM6-PACF-vgb and physicochemical characterization of produced chondroitin sulfate

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dc.contributor.authorErenler, Ayse Sebnem
dc.contributor.authorUnver, Tuba
dc.contributor.authorCeylan, Ahmet Faruk
dc.contributor.authorOzcan, Imren
dc.contributor.authorGeckil, Hikmet
dc.date.accessioned2026-04-04T13:19:00Z
dc.date.available2026-04-04T13:19:00Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractChondroitin Sulfate (CS) is an essential component of the extracellular matrix and is a sulfated glycosaminoglycan structurally composed of a polysaccharide chain consisting of N-acetyl galactosamine and glucuronic acid. The use of CS of animal origin is common in pharmacological research. The disadvantages of traditional sources and methods used in the production of CS, which is used in various applications in the medicine, veterinary, pharmacy, and cosmetic sectors, have made microbial production a vital alternative. In this study, recombinant Escherichia coli (pETM6-PACF-vgb) strain, in which kfoA, kfoC, kfoF and vgb gene regions are co-expressed, and E. coli pETM6-PACF strain, which does not contain the vgb gene, were used in the microbial production of CS. The vgb gene is the region responsible for expressing the bacterial protein Vitreoscilla hemoglobin (VtHb). This study investigated the effect of the expression of VtHb in E. coli on increasing bacterial cell respiration and, therefore, how ATP production would affect cell growth and the acquisition of chondroitin and microbial chondroitin sulfate (MCS) from biomass. The analysis results determined a 23.07% increase in the amount of MCS produced from the vgb+ strain. The presence of vgb had positively affected culture age and reproductive kinetics. Spectrophotometric measurements, NMR, HPLC, FT-IR, TGA, DTA, and DSC analyses for the reproductive values and physicochemical characterization of the obtained MCS were applied to discuss this production process. For more detailed results on this subject, future research focused on optimization is needed. © 2025 Cellular and Molecular Biology Association. All rights reserved.
dc.description.sponsorshipInönü Üniversitesi; IUBAP, (FYL-2020-1961)
dc.identifier.doi10.14715/cmb/2025.71.2.4
dc.identifier.endpage27
dc.identifier.issn0145-5680
dc.identifier.issue2
dc.identifier.pmid39976917
dc.identifier.scopus2-s2.0-85219161195
dc.identifier.scopusqualityQ4
dc.identifier.startpage21
dc.identifier.urihttps://doi.org/10.14715/cmb/2025.71.2.4
dc.identifier.urihttps://hdl.handle.net/11616/108090
dc.identifier.volume71
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherCellular and Molecular Biology Association
dc.relation.ispartofCellular and Molecular Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20250329
dc.subjectMicrobial biotechnology
dc.subjectMicrobial chondroitin
dc.subjectRecombinant bacteria
dc.subjectvgb gene
dc.subjectVitreoscilla hemoglobin
dc.titleEffect of vgb gene on microbial chondroitin sulfate production in recombinant Escherichia coli pETM6-PACF-vgb and physicochemical characterization of produced chondroitin sulfate
dc.typeArticle

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