The effects of phenoxybenzamine and cold stress on tyrosine hydroxylase enzyme activity and expression of tyrosine hydroxylase gene in the rat adrenal medulla
| dc.contributor.author | Yürekli, Muhittin | |
| dc.date.accessioned | 2021-10-19T09:01:23Z | |
| dc.date.available | 2021-10-19T09:01:23Z | |
| dc.date.issued | 1998 | |
| dc.department | İnönü Üniversitesi | en_US |
| dc.description.abstract | Abstract: Soguk uygulaması ve soguga ek olarak phenoxybenzamine uygulamasının, tirozin hidroksilaz (TH) ve TH mRNA seviyesi üzerindeki etkileri Sprague Dawley (SD) sıçanlarının adrenal medullasında araştırıldı. SD sıçanları 48 saat 4 °C’de soguga maruz bırakıldı. Phenoxybenzamine uygulaması % 0.9 NaCl içeren % 5 etanol içinde hazırlanarak 20 mg/kg olacak sekilde periton içine (i.p) yapıldı. TH aktivitesi, | en_US |
| dc.description.abstract | Abstract: The effects of cold exposure and cold plus phenoxybenzamine treatment on tyrosine hydroxylase (TH) activity and TH m RNA levels were investigated in the adrenal medulla of Sprague Dawley (SD) rats. SD rats were exposed to cold (4 °C) for 48 hours. Phenoxybenzamine was injected intraperitoneally (i.p) at 20 mg/kg, which was prepared in 5% ethanol containing 0.9 % NaCL TH activity was measured by monitoring the formation of 3H2O as a product of L?(3H) -tyrosine hydroxylation, the formation of L?(3H) -dopa from L?(3H) -tyrosine. Total adrenal RNA was isolated and blotted onto a nylon membrane and the immobilized RNA was then hybridized from TH cDNA probe. TH mRNA was assayed by densitometric scanning of the autoradiograms with a videodensitometer. TH activity and mRNA levels were found to be significantly increased in the adrenal medulla of SD rats exposed to cold for 48 hours and treated with cold plus phenoxybenzamine (P<0.01). | en_US |
| dc.identifier.citation | YÜREKLİ M (1998). The effects of phenoxybenzamine and cold stress on tyrosine hydroxylase enzyme activity and expression of tyrosine hydroxylase gene in the rat adrenal medulla. Turkish Journal of Biology, 22(2), 197 - 203. | en_US |
| dc.identifier.endpage | 203 | en_US |
| dc.identifier.issn | 1300-0152 | |
| dc.identifier.issn | 1303-6092 | |
| dc.identifier.issue | 2 | en_US |
| dc.identifier.startpage | 197 | en_US |
| dc.identifier.trdizinid | 24604 | en_US |
| dc.identifier.uri | https://hdl.handle.net/11616/42320 | |
| dc.identifier.uri | https://search.trdizin.gov.tr/yayin/detay/24604 | |
| dc.identifier.volume | 22 | en_US |
| dc.indekslendigikaynak | TR-Dizin | en_US |
| dc.language.iso | tr | en_US |
| dc.relation.ispartof | Turkish Journal of Biology | en_US |
| dc.relation.publicationcategory | Makale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.title | The effects of phenoxybenzamine and cold stress on tyrosine hydroxylase enzyme activity and expression of tyrosine hydroxylase gene in the rat adrenal medulla | en_US |
| dc.type | Article | en_US |
