Determination of Regional Intestinal Permeability of Diclofenac and Metoprolol Using a Newly-Developed and Validated High Performance Liquid Chromatographic Method

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dc.authoridKAYNAK, Mustafa Sinan/0000-0003-2917-2407
dc.authoridBuyuktuncel, Ebru/0000-0001-7269-6594
dc.authorwosidBuyuktuncel, Saliha Ebru/ABH-9343-2020
dc.authorwosidKAYNAK, Mustafa Sinan/D-9453-2019
dc.contributor.authorKaynak, Mustafa Sinan
dc.contributor.authorBuyuktuncel, Ebru
dc.contributor.authorCaglar, Hatice
dc.contributor.authorSahin, Selma
dc.date.accessioned2024-08-04T20:40:02Z
dc.date.available2024-08-04T20:40:02Z
dc.date.issued2015
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPurpose: To develop a simple and rapid reversed-phase high performance liquid chromatographic (HPLC) method with UV detection for the simultaneous determination of diclofenac, metoprolol tartrate, phenol red and propyl paraben in intestinal segments. Methods: The mobile phase consisted of 55% methanol, 45% of 12.5 mM potassium dihydrogen phosphate (KH2PO4) aqueous solution, adjusted to pH 7.0 using 0.2 N sodium hydroxide (NaOH) solution, and to which 0.3% (v/v) triethylamine was added. Analysis was run at a flow rate of 1.0 mL/min with a 12 min total run time at ambient temperature. The developed method was successfully applied to determination of the analytes in samples obtained from in situ single pass intestinal perfusion (SPIP) studies. Results: The calibration curves were linear for all compounds (r > 0.999) with a limit of detection (LOD) of 0.005, 0.1, 0.075 mu g/mL, and limit of quantification of 0.1, 0.3, 0.2 mu g/mL for metoprolol tartrate, phenol red and diclofenac respectively. The coefficient of variation for intra-day and inter-day precision was < 5% and accuracy was between 98 and 102%. Based on SPIP and HPLC studies, the estimated mean permeability in jejunum, ileum and colon was 0.319 +/- 0.184, 0.639 +/- 0.241 and 0.84 3 +/- 0.517 x 10(-4) cm/sec, respectively, for metoprolol tartrate while the corresponding permeability values were 1.585 +/- 0.729, 1.154 +/- 0.433 and 1.775 +/- 1.576 x 10(-4) cm/sec for diclofenac. Conclusion: The findings indicate that diclofenac is a highly permeable compound and its absorption occurs throughout the intestinal tract. Furthermore, the developed method is suitable for the analysis of diclofenac and metoprolol in intestinal regions.en_US
dc.identifier.doi10.4314/tjpr.v14i1.23
dc.identifier.endpage170en_US
dc.identifier.issn1596-5996
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84922013974en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage163en_US
dc.identifier.urihttps://doi.org/10.4314/tjpr.v14i1.23
dc.identifier.urihttps://hdl.handle.net/11616/96672
dc.identifier.volume14en_US
dc.identifier.wosWOS:000350646200023en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPharmacotherapy Groupen_US
dc.relation.ispartofTropical Journal of Pharmaceutical Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiopharmaceutics classification systemen_US
dc.subjectDiclofenacen_US
dc.subjectMetoprolol tartrateen_US
dc.subjectSegmental permeabilityen_US
dc.subjectIntestinal absorptionen_US
dc.subjectValidationen_US
dc.titleDetermination of Regional Intestinal Permeability of Diclofenac and Metoprolol Using a Newly-Developed and Validated High Performance Liquid Chromatographic Methoden_US
dc.typeArticleen_US

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