Capsular Polysaccharide Biosynthesis from Recombinant E. coli and Chondroitin Sulfate Production

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dc.contributor.authorErenler, Ayse Sebnem
dc.date.accessioned2024-08-04T20:46:45Z
dc.date.available2024-08-04T20:46:45Z
dc.date.issued2019
dc.departmentİnönü Üniversitesien_US
dc.description.abstractChondroitin sulfate (CS) is an important biomedical product. CS is the basic structural component of the mammalian extracellular matrix and is widely used in many applications in the fields of medicine, veterinary medicine, pharmaceuticals and cosmetics. For CS production, mainly animal sources are used. However, in today's conditions, due to various risks and artificial synthesis, there has been an increase in alternative sources of production methods for CS, instead of using animal resources. In this study as a powerful alternative microbial production of CS has been targeted. By using recombinant E. coli strains to integrate VHb/vgb(+) and kfo(+) systems, the aim was to obtain high purity CS from reliable biotechnological processes. Plasmid pUC8:15 bearing the vgb gene region, and plasmid pETM6-PACF carrying the kfoA, kfoC and kfoF genes responsible for chondroitin synthesis, were transferred to E. coli bacteria. Microbial CS was obtained by adding sulfate groups to chondroitin acquired after the treatments. The results were confirmed by HPLC and NMR analyses. The product, compared to its counterparts, was found to be an effective drug, potentially with a low molecular weight value.en_US
dc.description.sponsorshipTUBITAK 1001 [215S-861]en_US
dc.description.sponsorshipThis study was supported by TUBITAK 1001 (215S-861).en_US
dc.identifier.doi10.14715/cmb/2019.65.6.4
dc.identifier.endpage21en_US
dc.identifier.issn0145-5680
dc.identifier.issn1165-158X
dc.identifier.issue6en_US
dc.identifier.pmid31472043en_US
dc.identifier.scopus2-s2.0-85071752903en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage17en_US
dc.identifier.urihttps://doi.org/10.14715/cmb/2019.65.6.4
dc.identifier.urihttps://hdl.handle.net/11616/98943
dc.identifier.volume65en_US
dc.identifier.wosWOS:000478777000003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherC M B Assocen_US
dc.relation.ispartofCellular and Molecular Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChondroitinen_US
dc.subjectNonfructosylated chondroitinen_US
dc.subjectChondroitin sulfateen_US
dc.subjectVitreoscilla hemoglobinen_US
dc.subjectvgb geneen_US
dc.titleCapsular Polysaccharide Biosynthesis from Recombinant E. coli and Chondroitin Sulfate Productionen_US
dc.typeArticleen_US

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