Investigation of obesogenic effects of hexachlorobenzene, DDT and DDE in male rats
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Press Inc Elsevier Science
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Obesity has become a very important public health problem and is increasing globally. Genetics, individual and environmental factors play roles in the etiology of this complex disorder. Recently, several environmental pol-lutants have been suggested to have obesogenic activities. Peroxisome proliferator activating receptor gamma (PPAR gamma), uncoupling protein-1 (UCP1) and their expression in white adipose tissue (WAT) and brown adipose tissue (BAT) play key roles in adipogenesis. UCP3 and irisin were reported to play roles in non-shivering ther-mogenesis. Our primary aim was to investigate obesogenic effects of hexachlorobenzene (HCB), dichlor-odiphenyltrichloroethane (DDT) and dichlorodiphenyldichloroethylene (DDE) in rats. In addition, thermoregulatory effects of HCB, DDT and DDE were also investigated by analyzing the levels of Ucp3 and irisin. Thirty-two adult male Sprague-Dawley rats were randomly divided into four groups as control, HCB, DDT and DDE. Animals were administered with organochlorine pesticides (OCPs; 5 mg/kg bw) by oral gavage every other day for five weeks. At the end of the experimental period, the animals were sacrificed, BAT and WAT samples were collected to analyze Ppar gamma, Ucp1 and Ucp3 levels. Moreover, skeletal muscle samples were collected to examine Ucp3 and irisin levels. Serum glucose, cholesterol and triglyceride levels were also determined. Body weight and core temperature of the animals were not significantly affected by any of the OCP administration. Serum glucose, cholesterol and triglyceride levels were similar among the experimental groups. Ppar gamma expression was significantly elevated by HCB administration only in WAT (p < 0.05). On the other hand, both Ppar gamma and Ucp1 expressions were diminished in WAT and BAT (p < 0.01) by DDT treatment, while in WAT, DDE signifi-cantly decreased Ppar gamma expression without altering its expression in BAT (p < 0.001). Ucp3 and irisin levels in skeletal muscle were not altered. Our findings show that both DDT and DDE reduce the browning of WAT by suppressing white adipocytes and thus may have obesogenic activity in male rats without altering thermoreg-ulation. In addition, HCB, DDT and DDE-induced alterations in expression of Ppar gamma and Ucp1 in WAT implicates differential regulation of adipogenic processes.
Açıklama
Anahtar Kelimeler
DDE, DDT, HCB, Obesogen, Ppar?, Ucp1, Ucp3, Irisin
Kaynak
General and Comparative Endocrinology
WoS Q Değeri
Q4
Scopus Q Değeri
Q1
Cilt
327
