Antiproliferative Properties and Evaluation of Antioxidant of Different Cornelian Cherry Genotypes and Analysis of Phenolic and Sugar Compounds by HPLC
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The aim of this study was to investigate the chemical composition (vitamin C, phenolic, and sugar compounds), the cyto -toxic effect on healthy (L-929) and lung cancer (A-549) cells, and the antioxidant capacity of fruits belong to thirteen cornelian cherry (Cornus mas L.) genotypes grown under the same conditions in Turkey. Fruit samples were extracted by the ASE technique. The chemical composition was analyzed by HPLC-DAD-RID. A reversed-phase Clipeus C18 reversed-pha - se column (250 mm × 4.6 mm, 5 ?m) were used. For gradient elution mobile phase A contained 4.5 % acetic acid in water; solution B acetonitrile were used as mobile phase with flow rate 1.0 mL/min. Antioxidant capacity, total phenolic, and total anthocyanin content were determined using spectrophotometric methods. Cytotoxic effects were evaluated by MTT assay in L-929 and A-549 cell lines for 48 h. No toxic effect of the fruit extracts was observed on L-929 healthy mouse fibroblast cells, while it was determined to reduce cell proliferation (approximately 50%) on A-549 lung cancer cells. The featured genotypes were 44-03, 44-20, 44-21, 77-09, and 44-21, 44-16, 77-05, respectively. The featured genotypes for antioxidant capacity and cytotoxic effects on A-549 cells were 44-03, 44-20, 44-21, 77-09, and 44-21, 44-16, 77-05, respectively. The results have brought out that there are significant differences between the genotypes (p ? 0.05) and cornelian cherry fruits have a significant antioxidant capacity and potential for antiproliferative effects.
Açıklama
Anahtar Kelimeler
Kaynak
Hacettepe Journal of Biology and Chemistry
WoS Q Değeri
Scopus Q Değeri
Cilt
51
Sayı
1
