Inhibition of NADPH oxidase by apocynin promotes myocardial antioxidant response and prevents isoproterenol-induced myocardial oxidative stress in rats

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dc.authoridVardı, Nigar/0000-0003-0576-1696
dc.authoridTANBEK, Kevser/0000-0003-2099-2273
dc.authoridOzhan, Onural/0000-0001-9018-7849
dc.authoridYildiz, Azibe/0000-0001-5686-7867
dc.authoridPolat, Alaadin/0000-0002-6920-3856
dc.authoridTanbek, Kevser/0000-0003-2099-2273
dc.authoridTanriverdi, Lokman Hekim/0000-0003-4263-5234
dc.authorwosidVardı, Nigar/C-9549-2018
dc.authorwosidTANBEK, Kevser/ITR-9264-2023
dc.authorwosidOzhan, Onural/AAE-2356-2020
dc.authorwosidYildiz, Azibe/ABI-7998-2020
dc.authorwosidPolat, Alaadin/AAA-7171-2021
dc.authorwosidTanbek, Kevser/ABI-1174-2020
dc.authorwosidTanriverdi, Lokman Hekim/M-5701-2015
dc.contributor.authorTanriverdi, Lokman H.
dc.contributor.authorParlakpinar, Hakan
dc.contributor.authorOzhan, Onural
dc.contributor.authorErmis, Necip
dc.contributor.authorPolat, Alaadin
dc.contributor.authorVardi, Nigar
dc.contributor.authorTanbek, Kevser
dc.date.accessioned2024-08-04T20:44:01Z
dc.date.available2024-08-04T20:44:01Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPreventive and/or therapeutic interventions for ischemic heart disease have gained considerable attention worldwide. We investigated the mechanism(s) underlying cardioprotection of apocynin (APO) and whether it attenuates isoproterenol (ISO)-induced myocardial damage in vivo. Thirty-two male Wistar Albino rats were randomised into four groups (n = 8 for each group): Group I (Control); Group II (ISO), ISO was given intraperitoneally (ip) (150 mg/kg/d) daily for 2 consecutive days; Group III (APO+ISO), APO was applied ip 20 mg/kg 30 min before the first ISO administration and continued for the next 2 d after the second ISO administration; Group IV (ISO+APO), after the ISO treatment on days 1 and 2, 20 mg/kg APO was given ip on days 3 and 4. Cardioprotective effects of APO were evaluated by biochemical values, histopathological observations and the antiapoptotic relative proteins. Mean blood pressure, heart rate, and electrocardiography (ECG) were also monitored. Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), total oxidant status (TOS), total antioxidant capacity (TAC), oxidative stress index (OSI), caspase-3 and connexin 43 levels were determined. Major ECG changes were observed in the ISO-treated rats. MDA, TOS, OSI and creatine kinase levels decreased and SOD, CAT, GSH and TAC levels increased, indicating that APO reduced cardiac injury and oxidative stress compared with controls. APO also decreased the number of cardiomyocytes with pyknotic nuclei, inflammatory cell infiltration, intracytoplasmic vacuolisation and myofibrils. APO provides preventive and therapeutic effects on ISO-induced myocardial injury in rats by inhibiting reactive oxygen species production, blocking inflammation and enhancing antioxidant status.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey [1919B011302847]en_US
dc.description.sponsorshipThis research is supported by a research grant from the Scientific and Technological Research Council of Turkey (Reference no. 1919B011302847 belongs to Dr. Tanriverdi).en_US
dc.identifier.doi10.1080/10715762.2017.1375486
dc.identifier.endpage786en_US
dc.identifier.issn1071-5762
dc.identifier.issn1029-2470
dc.identifier.issue9-10en_US
dc.identifier.pmid28969461en_US
dc.identifier.scopus2-s2.0-85030533636en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage772en_US
dc.identifier.urihttps://doi.org/10.1080/10715762.2017.1375486
dc.identifier.urihttps://hdl.handle.net/11616/97985
dc.identifier.volume51en_US
dc.identifier.wosWOS:000415660400003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofFree Radical Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApocyninen_US
dc.subjectisoproterenolen_US
dc.subjectmyocardial infarctionen_US
dc.subjectoxidative stressen_US
dc.subjectratsen_US
dc.titleInhibition of NADPH oxidase by apocynin promotes myocardial antioxidant response and prevents isoproterenol-induced myocardial oxidative stress in ratsen_US
dc.typeArticleen_US

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