The synthesis of indole-3-acetic acid by the industrially important white-rot fungus Lentinus sajor-caju under different culture conditions
Küçük Resim Yok
Tarih
2003
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Cambridge Univ Press
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The synthesis of plant growth hormone auxin, indole-3-acetic acid (IAA), is not only confined to flowering plants. Yeasts and other fungi are also known to produce this hormone and in many cases at higher levels than plants. The main concern of this study was to determine the physical and chemical conditions necessary for optimal biosynthesis of this hormone by Lentinus sajor-caju. Glucose was determined to be superior to sucrose as carbon and energy source. The synthesis of IAA in a nitrogen-free medium or in a medium with low external phosphate was substantially reduced. Light exposed and non-agitated cultures grown in dark had also reduced levels of IAA compared to agitated cultures grown in dark. The highest (0.18 mg ml(-1)) IAA level was determined in cultures grown in glucose containing medium (pH 7.5) on a rotary shaker (150 rpm) at 30 degreesC in dark. The biological activity of IAA obtained from the extra-cellular culture of Lentinus sajor-caju was determined using oat coleptile growth test.
Açıklama
Anahtar Kelimeler
Abscisic-Acid, Gibberella-Fujikuroi, Indoleacetic-Acid, Sucrose, Zeatin
Kaynak
Mycological Research
WoS Q Değeri
Q2
Scopus Q Değeri
N/A
Cilt
107
