Sisplatinin böbrek metabolizması üzerine etkileri: Gingko biloba ekstraktının koruyuculuğu
Küçük Resim Yok
Tarih
2004
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Öz: Bu çalışmada, etkili bir antitümör ajan olan sisplatinin sıçanlarda böbrek metabolizmasının bozulmasına neden olup olmadığı ve Ginkgo biloba ekstraktı (GBE) ile bu bozukluğun önlenip önlenemeyeceğinin araştırılması amaçlandı. Deney grupları; kontrol grubu, Sisplatin grubu ve Sisplatin+Ginkgo biloba ekstraktı (Sisplatin+GBE) grubu şeklinde düzenlendi. Sisplatin ve Sisplatin+GBE grubundaki sıçanlara çalışmanın 4. gününde bir defa 7 mg/kg sisplatin intraperitoneal (i.p.) olarak verildi. Sisplatin+GBE grubunda, sisplatin verilmeden üç gün önce ve 7 gün sonra, GBE 100 mg/kg dozunda disposibl enjektör ucuna takılan kanül yardımıyla doğrudan mideye verildi. Kontrol grubundaki sıçanlara salin solusyonu verildi. Sisplatin uygulamasından 7 gün sonra böbrek dokuları alınarak supernatanda hekzokinaz (HK), glukoz-6-fosfat dehidrogenaz (G6PD), laktat dehidrogenaz (LDH) ve malat dehidrogenaz (MDH) enzim aktiviteleri ölçüldü. Kontrol grubu ile sisplatin grubu karşılaştırıldığında HK, G6PD, LDH ve MDH aktivitelerinde anlamlı bir artma gözlendi. Sisplatin+GBE grubunda kontrol ve sisplatin gruplarına göre G6PD aktivitelerinde anlamlı bir artma gözlendi. Sisplatin+GBE grubunda sisplatin grubuna göre LDH aktivitesinde anlamlı bir azalma gözlendi. Kontrol grubu ile Sisplatin+GBE grubu karşılaştırıldığında HK aktivitesinde anlamlı bir artma bulundu. Sisplatinin böbrek hücrelerinin metabolizmasını değiştirerek hasarı hızlandırabileceği, GBE'nın ise bu hasara karşı koruyucu etki gösterebileceği sonucuna varıldı.
Abstract: In this study, we aimed to examine whether antitumour drug cisplatin affect the metabolism of the kidney and consequently causes kidney damage and, Ginkgo biloba extract prevents this damage in rats. The experimental groups were as follows: Control group, Cisplatin-treated group (Cisplatin), and a group treated with Cisplatin plus Ginkgo biloba extract (GBE). The Cisplatin and Cisplatin+GBE groups were treated intraperitoneally with a single dose of 7 mg/kg body weight sisplatin at 4th day of the treatment. GBE was administrated at a dose of 100 mg/kg body weight orally, three days before and seven days after the treatment with cisplatin in the cisplatin+GBE group. Kidney tissues were taken 7 day after the cisplatin administration. The activities of kidney hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD), lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) serum enzymes were determined in each sample. The results of the experiment demonstrated that HK, G6PD, LDH, and MDH activities were increased significantly in the cisplatin group compared with control group. HK and G6PD activities were increased significantly in the cisplatin group compared with control group. G6PD activity was increased significantly in the cisplatin+GBE group compared with cisplatin group, and LDH activity was decreased significantly in the cisplatin+GBE group compared with cisplatin group. From these results, it can be concluded that cisplatin may cause kidney damage through metabolic enzymes and, GBE may prevent damage caused by cisplatin.
Abstract: In this study, we aimed to examine whether antitumour drug cisplatin affect the metabolism of the kidney and consequently causes kidney damage and, Ginkgo biloba extract prevents this damage in rats. The experimental groups were as follows: Control group, Cisplatin-treated group (Cisplatin), and a group treated with Cisplatin plus Ginkgo biloba extract (GBE). The Cisplatin and Cisplatin+GBE groups were treated intraperitoneally with a single dose of 7 mg/kg body weight sisplatin at 4th day of the treatment. GBE was administrated at a dose of 100 mg/kg body weight orally, three days before and seven days after the treatment with cisplatin in the cisplatin+GBE group. Kidney tissues were taken 7 day after the cisplatin administration. The activities of kidney hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD), lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) serum enzymes were determined in each sample. The results of the experiment demonstrated that HK, G6PD, LDH, and MDH activities were increased significantly in the cisplatin group compared with control group. HK and G6PD activities were increased significantly in the cisplatin group compared with control group. G6PD activity was increased significantly in the cisplatin+GBE group compared with cisplatin group, and LDH activity was decreased significantly in the cisplatin+GBE group compared with cisplatin group. From these results, it can be concluded that cisplatin may cause kidney damage through metabolic enzymes and, GBE may prevent damage caused by cisplatin.
Açıklama
Anahtar Kelimeler
Kaynak
Türk Hematoloji Onkoloji Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
14
Sayı
1
Künye
YILMAZ H. R,IŞIK B,GÜLEÇ M,SÖGÜT S,AKYOL Ö (2004). Sisplatinin böbrek metabolizması üzerine etkileri: Gingko biloba ekstraktının koruyuculuğu. Türk Hematoloji Onkoloji Dergisi, 14(1), 20 - 24.
