Local ADSC Delivery Methods Accelerate Healing of Large Unburned Full-Thickness Skin Defects by Promoting an Optimal Wound Microenvironment

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dc.contributor.authorGurunluoglu, Semra
dc.contributor.authorSatilmis, Basri
dc.contributor.authorGul, Mehmet
dc.contributor.authorDundar, Muhammed
dc.contributor.authorGurunluoglu, Kubilay
dc.contributor.authorKaraaslan, Ezgi
dc.contributor.authorKoc, Ahmet
dc.date.accessioned2026-04-04T13:31:12Z
dc.date.available2026-04-04T13:31:12Z
dc.date.issued2026
dc.departmentİnönü Üniversitesi
dc.description.abstractBackground: This study introduces an experimental model of a large, full-thickness skin defect and evaluates how adipose-derived stem cells characterized by high self-renewal and differentiation capacity affect both wound healing and the wound microenvironment when delivered using two different local application methods. Materials and Methods: In this preclinical study, we established an excisional full-thickness skin defect model involving approximately 30% of the total body surface area (TBSA). Five experimental groups were formed, each containing equal numbers of male and female rats: (1) subdermal ADSC injection (ADSC-I) (n = 8), (2) application of an acellular dermal matrix (ADM) seeded with ADSCs (n = 8) (ADSC-ADM), (3) ADM alone (n = 8), (4) subdermal saline injection (n = 8) (SS-I), and (5) an untreated skin-defect sham group (n = 8). Wound healing and wound microenvironment parameters were assessed at regular intervals using macroscopic and microscopic evaluations, as well as various quantitative measurements. The study was terminated when complete wound closure was achieved in all animals of at least one experimental group. Results: The most favorable healing outcomes were observed in the two ADSC-treated groups. More favorable microenvironmental conditions in the stem cell groups were detected from day 14 onward. Complete closure of the dermal defects occurred by day 32 in the ADSC-I group, whereas none of the other groups achieved full wound closure within the study period. Conclusions: Local application of adipose-derived stem cells may accelerate wound healing by favorably modulating the wound microenvironment.
dc.description.sponsorshipInonu University [TOA-2024-3403]
dc.description.sponsorshipThis work was supported by Inonu University Scientific Research Project Coordination Unit (project code: TOA-2024-3403).
dc.identifier.doi10.3390/biom16020320
dc.identifier.issn2218-273X
dc.identifier.issue2
dc.identifier.orcid0000-0002-9737-859X
dc.identifier.pmid41750388
dc.identifier.scopus2-s2.0-105031395897
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3390/biom16020320
dc.identifier.urihttps://hdl.handle.net/11616/108647
dc.identifier.volume16
dc.identifier.wosWOS:001700598400001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofBiomolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectwound healing
dc.subjectwound microenvironment
dc.subjectadipose-derived stem cells
dc.subjectacellular dermal matrix
dc.subjectfull-thickness skin defect
dc.titleLocal ADSC Delivery Methods Accelerate Healing of Large Unburned Full-Thickness Skin Defects by Promoting an Optimal Wound Microenvironment
dc.typeArticle

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