Ratlarda bleomisin ile oluşturulan akciğer fibrozisinded vitamini takviyesi ve yüzme egzersizinin antiinflamatuar ve antifibrotik etkisi
Küçük Resim Yok
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışma ile deneysel pulmoner fibrozis'de; D vitamini takviyesi ile fibrozis oluşumu ve gelişen inflamasyonu azaltmak hedeflenmektedir. Materyal ve Metot: Çalışmada 69 adet wistar albino rat kullanıldı. Bu ratlar, rastgele olacak şekilde toplam 10 gruba ayrıldı. Çalışmada deneysel pulmoner fibrozisi oluşturmak için, 2,5mg/kg BLM tek doz intratracheal olarak uygulandı. D vitamini 200 IU ve 100 IU olarak zeytinyağı içerisinde 6 hafta boyunca ratlara oral yoldan (gavaj) verildi. Ratlara, modifiye bir tank içerisinde, uygun sıcaklıkta (31±1 °C) her gün yüzme egzersizi yaptırıldı. BLM uygulamasından 28 gün sonra ratlar sakrifiye edildi. Ratlardan kan, BAL (bronkoalveolar lavaj) ve patoloji örnekleri alındı. Bulgular: BLM uygulanan grupta, serum TGF- ? değerinde kontrol grubuna göre anlamlı artış vardı (p<0,0011). BLM gurubuna göre, BLM+D vit200IU(5µg)+yüzme grubunun serum TGF- ? değerindeki azalma istatistiksel olarak anlamlıydı (p<0,0011). Fibrozis skorlamasında dağılımsal farklılık istatistiksel olarak anlamlı olup, BLM grubu en yüksek fibrozis skoruna sahipti. BLM grubuna göre, BLM+D vit200IU(5µg)+yüzme grubunda, şiddetli makrofaj daha az görülmüştür. Sonuç: Deneysel olarak oluşturduğumuz pulmoner fibrozis modelinde, uyguladığımız D vitamini ve yüzme egzersizi bulgularına göre, pulmoner fibrozis'de gerileme ve enflamasyonda azalma kaydettik. Bu çalışmanın klinikte uygulanması sonrasında, İPF'li bireylerde de araştırmamız paralelinde prognoza ve yaşam kalitesine olumlu katkı sağlayacağını düşünmekteyiz.
Aim: With this study, in experimental pulmonary fibrosis; Vitamin D supplementation aims to reduce fibrosis formation and developing inflammation Material and Method: 69 Wistar albino rats were used in the study. These rats were randomly divided into 10 groups in total. To create experimental pulmonary fibrosis in the study, a single dose of 2.5mg/kg BLM was administered intratracheally. Vitamin D was given orally (gavage) to rats as 200 IU and 100 IU in olive oil for 6 weeks. Rats were given swimming exercises every day in a modified tank at an appropriate temperature (31±1 °C). The rats were sacrificed 28 days after BLM application. Blood, BAL (bronchoalveolar lavage) and pathology samples were taken from the rats. Results: There was a significant increase in serum TGF-? value in the BLM applied group compared to the control group (p<0.0011). Compared to the BLM group, the decrease in serum TGF-? value of the BLM+D vit 200IU(5µg)+swimming group was statistically significant (p<0.0011). The distributional difference in fibrosis scoring was statistically significant, and the BLM group had the highest fibrosis score. Compared to the BLM group, severe macrophages were seen less in the BLM+D vit 200IU(5µg)+swimming group. Conclusion: In the pulmonary fibrosis model we created experimentally, we recorded a regression in pulmonary fibrosis and a decrease in inflammation, according to the vitamin D and swimming exercise findings. We think that after this study is implemented in the clinic, it will contribute positively to the prognosis and quality of life in individuals with IPF, in parallel with our research.
Aim: With this study, in experimental pulmonary fibrosis; Vitamin D supplementation aims to reduce fibrosis formation and developing inflammation Material and Method: 69 Wistar albino rats were used in the study. These rats were randomly divided into 10 groups in total. To create experimental pulmonary fibrosis in the study, a single dose of 2.5mg/kg BLM was administered intratracheally. Vitamin D was given orally (gavage) to rats as 200 IU and 100 IU in olive oil for 6 weeks. Rats were given swimming exercises every day in a modified tank at an appropriate temperature (31±1 °C). The rats were sacrificed 28 days after BLM application. Blood, BAL (bronchoalveolar lavage) and pathology samples were taken from the rats. Results: There was a significant increase in serum TGF-? value in the BLM applied group compared to the control group (p<0.0011). Compared to the BLM group, the decrease in serum TGF-? value of the BLM+D vit 200IU(5µg)+swimming group was statistically significant (p<0.0011). The distributional difference in fibrosis scoring was statistically significant, and the BLM group had the highest fibrosis score. Compared to the BLM group, severe macrophages were seen less in the BLM+D vit 200IU(5µg)+swimming group. Conclusion: In the pulmonary fibrosis model we created experimentally, we recorded a regression in pulmonary fibrosis and a decrease in inflammation, according to the vitamin D and swimming exercise findings. We think that after this study is implemented in the clinic, it will contribute positively to the prognosis and quality of life in individuals with IPF, in parallel with our research.
Açıklama
Anahtar Kelimeler
Anatomi, Anatomy
