Modulation of Neuronal Damage in DRG by Asprosin in a High-Glucose Environment and Its Impact on miRNA181-a Expression in Diabetic DRG

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dc.authoridKelestemur, Mirac/0000-0003-0455-4521
dc.authoridBILGIN, BATUHAN/0000-0002-3470-1783
dc.authoridADAM, Muhammed/0000-0002-5080-5160
dc.authorwosidKelestemur, Mirac/AAK-8577-2021
dc.authorwosidDalkılıç, Semih/GLV-3548-2022
dc.contributor.authorAdam, Muhammed
dc.contributor.authorOzcan, Sibel
dc.contributor.authorDalkilic, Semih
dc.contributor.authorTektemur, Nalan Kaya
dc.contributor.authorTekin, Suat
dc.contributor.authorBilgin, Batuhan
dc.contributor.authorHekim, Munevver Gizem
dc.date.accessioned2024-08-04T20:54:57Z
dc.date.available2024-08-04T20:54:57Z
dc.date.issued2024
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAsprosin, a hormone secreted from adipose tissue, has been implicated in the modulation of cell viability. Current studies suggest that neurological impairments are increased in individuals with obesity-linked diabetes, likely due to the presence of excess adipose tissue, but the precise molecular mechanism behind this association remains poorly understood. In this study, our hypothesis that asprosin has the potential to mitigate neuronal damage in a high glucose (HG) environment while also regulating the expression of microRNA (miRNA)-181a, which is involved in critical biological processes such as cellular survival, apoptosis, and autophagy. To investigate this, dorsal root ganglion (DRG) neurons were exposed to asprosin in a HG (45 mmol/L) environment for 24 hours, with a focus on the role of the protein kinase A (PKA) pathway. Expression of miRNA-181a was measured by using real-time polymerase chain reaction (RT-PCR) in diabetic DRG. Our findings revealed a decline in cell viability and an upregulation of apoptosis under HG conditions. However, pretreatment with asprosin in sensory neurons effectively improved cell viability and reduced apoptosis by activating the PKA pathway. Furthermore, we observed that asprosin modulated the expression of miRNA-181a in diabetic DRG. Our study demonstrates that asprosin has the potential to protect DRG neurons from HG-induced damage while influencing miRNA-181a expression in diabetic DRG. These findings provide valuable insights for the development of clinical interventions targeting neurotoxicity in diabetes, with asprosin emerging as a promising therapeutic target for managing neurological complications in affected individuals.en_US
dc.description.sponsorshipTrkiye Bilimsel ve Teknolojik Arascedil;timath;rma Kurumuen_US
dc.description.sponsorshipNo Statement Availableen_US
dc.identifier.doi10.1007/s12640-023-00678-9
dc.identifier.issn1029-8428
dc.identifier.issn1476-3524
dc.identifier.issue1en_US
dc.identifier.pmid38133838en_US
dc.identifier.scopus2-s2.0-85180492828en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s12640-023-00678-9
dc.identifier.urihttps://hdl.handle.net/11616/101738
dc.identifier.volume42en_US
dc.identifier.wosWOS:001127431900002en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofNeurotoxicity Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAsprosinen_US
dc.subjectDiabetesen_US
dc.subjectCell viabilityen_US
dc.subjectApoptosisen_US
dc.subjectDorsal root ganglionen_US
dc.subjectmiRNA-181aen_US
dc.titleModulation of Neuronal Damage in DRG by Asprosin in a High-Glucose Environment and Its Impact on miRNA181-a Expression in Diabetic DRGen_US
dc.typeArticleen_US

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