Investigation of the effects of pinealectomy on acetylcholinesterase enzyme activity and oxidative stress in rat brain

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dc.contributor.authorPekmez, Hidir
dc.contributor.authorOzkaya, Ahmet
dc.contributor.authorUckun, Mirac
dc.contributor.authorZayman, Emrah
dc.contributor.authorAydin, Merve
dc.contributor.authorCanpolat, Sinan
dc.contributor.authorGul, Mehmet
dc.date.accessioned2026-04-04T13:35:17Z
dc.date.available2026-04-04T13:35:17Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractThe pineal gland releases melatonin to regulate our body's circadian rhythm based on light and dark cycles. The pinealectomy (PINX) model is an experimental approach employed to investigate the potential impact of melatonin on various tissues and pathologies. In this study, acetylcholinesterase (AChE) enzyme activity levels, oxidative stress parameters, histopathological findings, and serum melatonin levels in rat brain tissue were evaluated following pinealectomy. 24 male Sprague Dawley rats were randomly divided into groups: control, Sham-Pinealectomy (SHAM), and PINX. Brain tissue samples were taken at the end of a 50-day experimental period to determine the parameters of AChE, glutathione s-transferase (GST), carboxylesterase (Ces) enzyme activities, and malondialdehyde (MDA), reduced glutathione (GSH) levels spectrophotometrically. Moreover, serum melatonin levels were measured, and tissues underwent standard histological analysis to determine the histopathological damage score. In this study, we found that the PINX group had decreased AChE and Ces enzyme activity, increased MDA, decreased GSH levels, and no change in GST enzyme activity. A relative decrease in serum melatonin levels was also observed in the PINX group. In the light microscopic examination of the brain tissue of pinealectomy rats, it was observed that the eosinophilic staining intensity increased, heterochromatic/pycnotic-looking neuron nuclei were prominent in the cortex layers and hippocampus, and perineural edematous areas were abundant. Excessive perineuronal edema, cytoplasmic eosinophilia, and heterochromatic/pycnotic nuclei were found based on the histopathological damage score. After pinealectomy, we observed an increase in oxidative stress and a decrease in AChE levels in the brain.
dc.identifier.doi10.1007/s41105-025-00576-x
dc.identifier.endpage293
dc.identifier.issn1446-9235
dc.identifier.issn1479-8425
dc.identifier.issue3
dc.identifier.orcid0000-0002-9018-8515
dc.identifier.pmid40538385
dc.identifier.scopus2-s2.0-85217737793
dc.identifier.scopusqualityQ3
dc.identifier.startpage283
dc.identifier.urihttps://doi.org/10.1007/s41105-025-00576-x
dc.identifier.urihttps://hdl.handle.net/11616/109740
dc.identifier.volume23
dc.identifier.wosWOS:001420005900001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Japan Kk
dc.relation.ispartofSleep and Biological Rhythms
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectPinealectomy
dc.subjectAChE
dc.subjectOxidative stress
dc.subjectMelatonin
dc.titleInvestigation of the effects of pinealectomy on acetylcholinesterase enzyme activity and oxidative stress in rat brain
dc.typeArticle

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