The investigation of the responses to phytohemagglutinin in the patients of non-Hodgkin's lymphoma who were candidates for high dose chemotherapy, autologous bone marrow or peripheral stem cell transplantation
Yükleniyor...
Dosyalar
Tarih
1996
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi Turgut Özal Tıp Merkezi Dergisi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Öz:Fitohemaglutinin (PHA), genellikle bir T lenfosit mitojenidir. CD4+ helper/inducer T lenfositlerine, CD8' suppressor/cytotoxic T lenfositlerine göre daha fazla stimulator etki yapar. Bu çalışmada, biz normal periferik kan lökositleri (PBL) ile otolog kemik iliği (BM) ve granulosit-monosit koloni stîmulan faktör (GM-CSF) ile mobilize edilmiş periferik stem cell (PSC) ürünlerinin PHA 'e verdikleri mitojenik cevapların farklı olup olmadığını araştırdık. Non-Hodgkin lenfomalı 24 hastadan kemik iliği ve periferik stem cell ürünleri toplandı ve IL-2 ile 5 gün kültür yapılmadan önce ve yapıldıktan sonra PHA hücre proliferasyon cevabı incelendi. Hem PSC hem de BM hücrelerinin PHA proliferatif cevapları anlamlı şekilde normal PBL 'den düşüktü (p<0.01). PSC'in PHA cevabı BM'unkinden daha yüksekti (p<0 05). IL-2 ile kültür yapıldıktan sonra PBL'nin PHA cevabı azaldı (p<0.05) fakat PSC ve BM'unki değişmedi. Bu çalışma sonucunda IL-2 ile inkübasyona PSC ürünleri PHA'e daha yüksek mitojenik cevap verdi. Bu sonuçlara göre non-Hodgkin lenfomalı hastaların lenfositlerindeki deprese mitojenik cevap, membrana bağlı IL-2'nin sürekli stimüle olması nedeniyle yüksek baz proliferasyona sebep olduğunu düşündürmektedir. [Turgut Özal Tıp Merkezi Dergisi 1996;3(4):306-310]
Abstract:Phytohemagglutinin (PHA) is principally a T lymphocyte mitogen, producing a greater stimulatory effect* on CD4+ helper/inducer T lymphocytes than on CD8+ suppressor/cytotoxic T cells. In this study, investigated if there are differences in the mitogenic response to PHA between autologous bone marrow " (BM) and granulocyte-monocyte colony stimulating factor (GM-CSF)-mobilized peripheral stem cell (PSC) products as well as to normal peripheral blood leukocytes (PBL). BM and PSC products from 24 patients with non-Hodgkin's lymphoma (NHL) were collected and assays of cell proliferation in response to PHA were performed both before and following 5 days co-incubation with IL^2. The proliferative response to PHA by cells from both PSC and BM products were significantly lower than normal PBL (p<0.00I). The PHA response of PSC was significantly higher than that observed with BM (p<0.01). The response to PHA of PSC was higher than BM (p<0.05). When compared before culture with IL-2, the PHA response of PBL was decreased (p<0.05), but PSC and BM was not changed. This study showed that the PSC products prior to IL-2 incubation had higher mitogenic response to PHA. According to these results the depressed mitogenic response of lymphocytes from non-Hodgkin patients was associated with an increase in "baseline" proliferation due to membrane bound IL-2 resulting in continued proliferation. [Journal of Turgut Özal-Medical.Centerl996;3(4):306-310]
Abstract:Phytohemagglutinin (PHA) is principally a T lymphocyte mitogen, producing a greater stimulatory effect* on CD4+ helper/inducer T lymphocytes than on CD8+ suppressor/cytotoxic T cells. In this study, investigated if there are differences in the mitogenic response to PHA between autologous bone marrow " (BM) and granulocyte-monocyte colony stimulating factor (GM-CSF)-mobilized peripheral stem cell (PSC) products as well as to normal peripheral blood leukocytes (PBL). BM and PSC products from 24 patients with non-Hodgkin's lymphoma (NHL) were collected and assays of cell proliferation in response to PHA were performed both before and following 5 days co-incubation with IL^2. The proliferative response to PHA by cells from both PSC and BM products were significantly lower than normal PBL (p<0.00I). The PHA response of PSC was significantly higher than that observed with BM (p<0.01). The response to PHA of PSC was higher than BM (p<0.05). When compared before culture with IL-2, the PHA response of PBL was decreased (p<0.05), but PSC and BM was not changed. This study showed that the PSC products prior to IL-2 incubation had higher mitogenic response to PHA. According to these results the depressed mitogenic response of lymphocytes from non-Hodgkin patients was associated with an increase in "baseline" proliferation due to membrane bound IL-2 resulting in continued proliferation. [Journal of Turgut Özal-Medical.Centerl996;3(4):306-310]
Açıklama
Yıl: 1996Cilt: 3Sayı: 4ISSN: 1300-1744Sayfa Aralığı: 306 - 310Metin Dili:İngilizce
Anahtar Kelimeler
Kaynak
İnönü Üniversitesi Turgut Özal Tıp Merkezi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
ÖZEROL İ. H,ŞENOL M,AGEITOS A,TALMADGE J. E (1996). The investigation of the responses to phytohemagglutinin in the patients of non-Hodgkin's lymphoma who were candidates for high dose chemotherapy, autologous bone marrow or peripheral stem cell transplantation. İnönü Üniversitesi Turgut Özal Tıp Merkezi Dergisi, 3(4), 306 - 310.
