The investigation of the responses to phytohemagglutinin in the patients of non-Hodgkin's lymphoma who were candidates for high dose chemotherapy, autologous bone marrow or peripheral stem cell transplantation
| dc.contributor.author | Özerol, İbrahim Halil | |
| dc.contributor.author | Şenol, Mustafa | |
| dc.contributor.author | Ageıtos, Ana | |
| dc.contributor.author | Talmadge, James E. | |
| dc.date.accessioned | 2020-08-26T13:15:16Z | |
| dc.date.available | 2020-08-26T13:15:16Z | |
| dc.date.issued | 1996 | |
| dc.department | İnönü Üniversitesi | en_US |
| dc.description | Yıl: 1996Cilt: 3Sayı: 4ISSN: 1300-1744Sayfa Aralığı: 306 - 310Metin Dili:İngilizce | en_US |
| dc.description.abstract | Öz:Fitohemaglutinin (PHA), genellikle bir T lenfosit mitojenidir. CD4+ helper/inducer T lenfositlerine, CD8' suppressor/cytotoxic T lenfositlerine göre daha fazla stimulator etki yapar. Bu çalışmada, biz normal periferik kan lökositleri (PBL) ile otolog kemik iliği (BM) ve granulosit-monosit koloni stîmulan faktör (GM-CSF) ile mobilize edilmiş periferik stem cell (PSC) ürünlerinin PHA 'e verdikleri mitojenik cevapların farklı olup olmadığını araştırdık. Non-Hodgkin lenfomalı 24 hastadan kemik iliği ve periferik stem cell ürünleri toplandı ve IL-2 ile 5 gün kültür yapılmadan önce ve yapıldıktan sonra PHA hücre proliferasyon cevabı incelendi. Hem PSC hem de BM hücrelerinin PHA proliferatif cevapları anlamlı şekilde normal PBL 'den düşüktü (p<0.01). PSC'in PHA cevabı BM'unkinden daha yüksekti (p<0 05). IL-2 ile kültür yapıldıktan sonra PBL'nin PHA cevabı azaldı (p<0.05) fakat PSC ve BM'unki değişmedi. Bu çalışma sonucunda IL-2 ile inkübasyona PSC ürünleri PHA'e daha yüksek mitojenik cevap verdi. Bu sonuçlara göre non-Hodgkin lenfomalı hastaların lenfositlerindeki deprese mitojenik cevap, membrana bağlı IL-2'nin sürekli stimüle olması nedeniyle yüksek baz proliferasyona sebep olduğunu düşündürmektedir. [Turgut Özal Tıp Merkezi Dergisi 1996;3(4):306-310] | en_US |
| dc.description.abstract | Abstract:Phytohemagglutinin (PHA) is principally a T lymphocyte mitogen, producing a greater stimulatory effect* on CD4+ helper/inducer T lymphocytes than on CD8+ suppressor/cytotoxic T cells. In this study, investigated if there are differences in the mitogenic response to PHA between autologous bone marrow " (BM) and granulocyte-monocyte colony stimulating factor (GM-CSF)-mobilized peripheral stem cell (PSC) products as well as to normal peripheral blood leukocytes (PBL). BM and PSC products from 24 patients with non-Hodgkin's lymphoma (NHL) were collected and assays of cell proliferation in response to PHA were performed both before and following 5 days co-incubation with IL^2. The proliferative response to PHA by cells from both PSC and BM products were significantly lower than normal PBL (p<0.00I). The PHA response of PSC was significantly higher than that observed with BM (p<0.01). The response to PHA of PSC was higher than BM (p<0.05). When compared before culture with IL-2, the PHA response of PBL was decreased (p<0.05), but PSC and BM was not changed. This study showed that the PSC products prior to IL-2 incubation had higher mitogenic response to PHA. According to these results the depressed mitogenic response of lymphocytes from non-Hodgkin patients was associated with an increase in "baseline" proliferation due to membrane bound IL-2 resulting in continued proliferation. [Journal of Turgut Özal-Medical.Centerl996;3(4):306-310] | en_US |
| dc.identifier.citation | ÖZEROL İ. H,ŞENOL M,AGEITOS A,TALMADGE J. E (1996). The investigation of the responses to phytohemagglutinin in the patients of non-Hodgkin's lymphoma who were candidates for high dose chemotherapy, autologous bone marrow or peripheral stem cell transplantation. İnönü Üniversitesi Turgut Özal Tıp Merkezi Dergisi, 3(4), 306 - 310. | en_US |
| dc.identifier.uri | https://hdl.handle.net/11616/17854 | |
| dc.identifier.uri | https://app.trdizin.gov.tr/publication/paper/detail/TkRFeE1UZzQ | |
| dc.language.iso | tr | en_US |
| dc.publisher | İnönü Üniversitesi Turgut Özal Tıp Merkezi Dergisi | en_US |
| dc.relation.ispartof | İnönü Üniversitesi Turgut Özal Tıp Merkezi Dergisi | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.title | The investigation of the responses to phytohemagglutinin in the patients of non-Hodgkin's lymphoma who were candidates for high dose chemotherapy, autologous bone marrow or peripheral stem cell transplantation | en_US |
| dc.title.alternative | Yüksek doz kemoterapi, otolog kemik iliği veya periferik stem cell transplantasyonu yapılacak non-Hodgkin lenfomalı hastalarda fitohemaglutinin'e cevabın araştırılması | en_US |
| dc.type | Article | en_US |
