Investigation of carbapenem resistance and the first identification of Klebsiella pneumoniae carbapenemase (KPC) enzyme among Escherichia coli isolates in Turkey: A prospective study

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dc.authoridKarakullukcu, Asiye/0000-0002-7117-5102
dc.authoridKarakullukcu, Asiye/0000-0002-7117-5102
dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authoridaygün, gökhan/0000-0001-6915-9843
dc.authoridKUSKUCU, MERT AHMET/0000-0001-8735-5725
dc.authoridMete, Bilgul/0000-0001-9091-6087
dc.authorwosidKarakullukcu, Asiye/F-4464-2019
dc.authorwosidKuskucu, Mert Ahmet/B-6373-2009
dc.authorwosidMete, Bilgül/A-6118-2019
dc.authorwosidaygün, gökhan/D-6627-2019
dc.authorwosidKarakullukcu, Asiye/KFS-0171-2024
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.authorwosidaygün, gökhan/IZP-8816-2023
dc.contributor.authorKuskucu, Mert Ahmet
dc.contributor.authorKarakullukcu, Asiye
dc.contributor.authorAiliken, Mailihaba
dc.contributor.authorOtlu, Bans
dc.contributor.authorMete, Bilgul
dc.contributor.authorAygun, Gokhan
dc.date.accessioned2024-08-04T20:42:52Z
dc.date.available2024-08-04T20:42:52Z
dc.date.issued2016
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground: The aim of this study was to determine the presence of carbapenem resistance and carbapenemase production in Escherichia coil isolates from clinical samples in Turkey. Methods: The prospective study included a total of 4.052 Escherichia coli isolates collected from patients admitted to a hospital from March 2011 to May 2012. We used ertapenem disc for screening carbapenemase production, and the confirmation was performed by using Etest. The resistance mechanisms and genetic relatedness of the carbapenem resistant strains were investigated by using PCR (polymerase chain reaction) and pulsed -field gel electrophoresis (PFGE), respectively. Results: Among the 4.052 E coli isolates, 24 (0.59%) were found to be carbapenem resistant. Of these, only 5 isolates were positive for OXA-48 and 2 isolates were positive for ICIebsiella pneumoniae carbapenemase (KPC)-2. The KPC-2 producing E. coil strains (n = 2) were both isolated from the same patient. The bla(KPC) genes were confirmed using DNA sequence analysis. The genetic relationship between the 24 E. coil strains studied by PFGE revealed that the strains were genetically unrelated. Conclusions: This article confirms, to our knowledge for the first time, the detection of KPC-2-producing E. coil in Turkey, with OXA-48 being the most frequent carbapenemase in the study. (C) 2016 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipResearch Found of Istanbul University [17546/2012]en_US
dc.description.sponsorshipThe study was partially funded by a project (no: 17546/2012) from the Research Found of Istanbul University and approved by the Ethics committees of Cerrahapasa Medical Faculty, Istanbul, Turkey. The preliminary data belongs this work was presented at the XXXV. Turkish Microbiology Congress, 3-7 November 2012, Kusadasi, Turkey (Oral presentation, hall A, no: S.4.1).en_US
dc.identifier.doi10.1016/j.tmaid.2016.11.006
dc.identifier.endpage576en_US
dc.identifier.issn1477-8939
dc.identifier.issn1873-0442
dc.identifier.issue6en_US
dc.identifier.pmid27890667en_US
dc.identifier.scopus2-s2.0-85006154105en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage572en_US
dc.identifier.urihttps://doi.org/10.1016/j.tmaid.2016.11.006
dc.identifier.urihttps://hdl.handle.net/11616/97633
dc.identifier.volume14en_US
dc.identifier.wosWOS:000391075400007en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofTravel Medicine and Infectious Diseaseen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEscherichia colien_US
dc.subjectCarbapenem resistanceen_US
dc.subjectCarbapenemaseen_US
dc.subjectKlebsiella pneumoniae carbapenemaseen_US
dc.subjectOXA-48en_US
dc.titleInvestigation of carbapenem resistance and the first identification of Klebsiella pneumoniae carbapenemase (KPC) enzyme among Escherichia coli isolates in Turkey: A prospective studyen_US
dc.typeArticleen_US

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