Pentylenetetrazole-induced kindling rat model: miR-182 and miR-27b-3p mediated neuroprotective effect of thymoquinone in the hippocampus

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dc.authoridPala, Mukaddes/0000-0002-0610-0526
dc.authoridTaslidere, Elif/0000-0003-1723-2556
dc.authoridokur, sema karaca/0000-0002-0692-8155
dc.authorwosidPala, Mukaddes/HKE-7637-2023
dc.authorwosidTaslidere, Elif/ABI-8046-2020
dc.authorwosidYılmaz, Şenay Görücü/A-3797-2016
dc.contributor.authorPala, Mukaddes
dc.contributor.authorMeral, Ismail
dc.contributor.authorAcikgoz, Nilgun Pala
dc.contributor.authorYilmaz, Senay Gorucu
dc.contributor.authorTaslidere, Elif
dc.contributor.authorOkur, Sema Karaca
dc.contributor.authorAcar, Seyma
dc.date.accessioned2024-08-04T20:51:46Z
dc.date.available2024-08-04T20:51:46Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjectives: Epilepsy is a neurological disease that pathologically affects brain functions. The epileptic hippocampus has modified microRNA(miRNA) levels. Therefore, we aimed to evaluate the neuroprotective effect of thymoquinone (TQ) in PTZ-induced epilepsy and to demonstrate the overlap between miRNA and mRNA expression profiles. Methods: Male adult Wistar albino rats (200-230 g, n = 20) were divided into three groups as control (n = 6), PTZ (n = 7), and TQ + PTZ (n = 7). The PTZ kindling model was created by injecting PTZ in sub convulsive doses to rats on days 1, 3, 5, 8, 10, 12, 15, 17, 19, 22, and 24 of the study into animals. Clonic and tonic seizures were induced by injecting a convulsive dose of PTZ on day 26 of the study. Rats in the TQ+PTZ group were treated by oral gavage with a 20 mg/kg TQ 2 h before each PTZ injection. The rats in the control group were treated with 0.5 ml saline. Seizure severity was evaluated with the Racine scale. The genes and signaling pathways targeted by miRNAs were determined by bioinformatics analysis. Results: In the rat hippocampus, mature 728 miRNAs were analyzed by microarray and the nine miRNA were verified by quantitative Real-Time PCR. rno-miR-182 and rno-miR-27b-3p were up-regulated in the PTZ group and down-regulated in the TQ + PTZ group. Discussion: In the PTZ kindling epilepsy model, the expression of these two miRNAs was regulated by TQ and exerted a neuroprotective effect by controlling the activities of target genes.en_US
dc.description.sponsorshipResearch Fund of Bezmialem Vakif University [12.2014/15]en_US
dc.description.sponsorshipThe present work was supported by the Research Fund of Bezmialem Vakif University, Project No. 12.2014/15.en_US
dc.identifier.doi10.1080/01616412.2022.2051129
dc.identifier.endpage737en_US
dc.identifier.issn0161-6412
dc.identifier.issn1743-1328
dc.identifier.issue8en_US
dc.identifier.pmid35282795en_US
dc.identifier.scopus2-s2.0-85126534372en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage726en_US
dc.identifier.urihttps://doi.org/10.1080/01616412.2022.2051129
dc.identifier.urihttps://hdl.handle.net/11616/100539
dc.identifier.volume44en_US
dc.identifier.wosWOS:000768059300001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofNeurological Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEpilepsyen_US
dc.subjectmiR-182en_US
dc.subjectmiR-27b-3pen_US
dc.subjectthymoquinoneen_US
dc.subjectpentilentetrazoleen_US
dc.titlePentylenetetrazole-induced kindling rat model: miR-182 and miR-27b-3p mediated neuroprotective effect of thymoquinone in the hippocampusen_US
dc.typeArticleen_US

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