Immobilization of glucose oxidase in silica sol-gel film for application to biosensor and amperometric determination of glucose

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dc.authorscopusid6504218851
dc.authorscopusid7005222498
dc.authorscopusid8261847900
dc.contributor.authorDemirkiran N.
dc.contributor.authorEkinci E.
dc.contributor.authorAsiltürk M.
dc.date.accessioned2024-08-04T19:59:31Z
dc.date.available2024-08-04T19:59:31Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIn this study, an electrochemical biosensor was developed by using a sol-gel coating solution. The modified platinum electrode used in the study was constructed by immobilization of glucose oxidase under a layer of sol-gel film. The sol-gel coating solution was prepared by using GLYMO, TEOS, and MTEOS. Electrochemical measurements were carried out amperometrically by determining hydrogen peroxide produced by the enzymatic reaction between glucose and glucose oxidase. The amperometric responses of the resulting enzymatic electrode to glucose were rapid. It was observed that the amperometric response of the enzymatic electrode was linear for glucose concentrations in the range from 2 to 18 mM with 50 s response time. LOD and LOQ for the enzymatic electrode were calculated to be 0.055 mM and 0.184 mM, respectively. It was determined that the developed biosensor had an acceptable reproducibility. The selectivity of the biosensor was determined in the presence of some interfering substances, such as lactose, sucrose, urea, uric acid, oxalic acid, and ascorbic acid. The stability of the biosensor was investigated, and it was found that the sensor response decreased by 59% of its initial response over a period of 30 days of storage in dry conditions at 4 °C.en_US
dc.identifier.endpage1339en_US
dc.identifier.issn0717-9324
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84877018385en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1336en_US
dc.identifier.urihttps://hdl.handle.net/11616/90683
dc.identifier.volume57en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofJournal of the Chilean Chemical Societyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmperometryen_US
dc.subjectBiosensoren_US
dc.subjectGlucoseen_US
dc.subjectGlucose oxidaseen_US
dc.subjectSol-gelen_US
dc.titleImmobilization of glucose oxidase in silica sol-gel film for application to biosensor and amperometric determination of glucoseen_US
dc.typeArticleen_US

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