Analysis of E6/E7 mRNA gene expression by means of NucliSENS EasyQ (NASBA) technique in patients suffering from cervical dysplasia with HPV positive

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dc.authoridCOSKUN, Ebru INCI/0000-0003-4402-3725
dc.authoridCiplak, Baris/0000-0002-9981-9264
dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authorwosidCOSKUN, Ebru INCI/ABI-8238-2020
dc.authorwosidTuncay, Görkem/ABI-6418-2020
dc.authorwosidCiplak, Baris/AAB-2086-2021
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.contributor.authorCiplak, B.
dc.contributor.authorYilmaz, E.
dc.contributor.authorOtlu, B.
dc.contributor.authorCoskun, E. I.
dc.contributor.authorSahin, N.
dc.contributor.authorTuncay, G.
dc.date.accessioned2024-08-04T20:44:08Z
dc.date.available2024-08-04T20:44:08Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjective: In this study, the authors aimed at examining E6/E7 mRNA gene expression by means of NucliSENS EasyQ (NASBA) in patients in which HPV infection was detected and diagnosed with cervical dysplasia. Materials and Methods: This study included 77 patients diagnosed with cervical dysplasia. The patients were grouped based on cervico-vaginal smear anomalies. Digene HC2 DNA Collection Device transport medium was used for taking and keeping cervical samples, QIA symphony SP device, and QIAsymphony DSP AXpH DNA kit were used for HPV DNA extraction from cervical samples kept in transport medium, HPV Q24 complete kit, RotorGene and PyroMark Q24 for detection of HPV-DNA and determination of HPV types and NucliSENS Easy Q Genetic Analyzer technique for determination of E6/E7 gene expression in HPV positive samples. SPSS (Statistical Package for Social Sciences) Version 22.0 was employed in statistical evaluation of the research date. Results: The most common HPV was considered as type 16 in this study. When pathology specimens of patients in whom dysplasia was detected in their cervical biopsies were examined in terms of HPV E6/E7 mRNA expression, a statistically significant difference was found between the normal and pathology groups (p < 0.05). Conclusion: Today, the presence of HPV infection in etiology of cervical smear pathologies and dysplasia is undisputedly accepted. The main determinant factor of HPV virulence is the frequency of E6 and E7 gene expressions existing in the DNA structure and responsible for virulence. NucliSENS_Ea_syQ (NASBA) is a technique employed to analyze mRNA gene expression in an accurate, reliable, and rapid way. Parallel to the conclusions of studies in the literature, frequency of E6/E7 gene expression increases in proportion to the increasing dysplasia degree of cervical pathologies in the present study as well.en_US
dc.identifier.doi10.12892/ejgo3620.2017
dc.identifier.endpage694en_US
dc.identifier.issn0392-2936
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85037041331en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage690en_US
dc.identifier.urihttps://doi.org/10.12892/ejgo3620.2017
dc.identifier.urihttps://hdl.handle.net/11616/98056
dc.identifier.volume38en_US
dc.identifier.wosWOS:000410875900007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisher7847050 Canada Incen_US
dc.relation.ispartofEuropean Journal of Gynaecological Oncologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCervical dysplasiaen_US
dc.subjectHPV, NucliSENS EasyQ (NASBA) techniqueen_US
dc.subjectE6/E7en_US
dc.titleAnalysis of E6/E7 mRNA gene expression by means of NucliSENS EasyQ (NASBA) technique in patients suffering from cervical dysplasia with HPV positiveen_US
dc.typeArticleen_US

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