Serotoninergic modulation in the brainstem and hypothalamus of female overnourished rats: impact on mitochondrial markers, oxidative stress and BDNF mRNA levels

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dc.contributor.authorRodrigues, Thyago de Oliveira
dc.contributor.authordos Santos Junior, Osmar Henrique
dc.contributor.authorBeltrao de Lemos, Maria Daniele Teixeira
dc.contributor.authorde Sousa Fernandes, Matheus Santos
dc.contributor.authorYagin, Fatma Hilal
dc.contributor.authorYagin, Burak
dc.contributor.authorDas, Samarjit
dc.date.accessioned2026-04-04T13:31:18Z
dc.date.available2026-04-04T13:31:18Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractIntroduction Obesity is a global epidemic identified by the World Health Organization, and its complexity involves genetic, cultural, socioeconomic, and behavioral factors.Methods In this study, we used female Wistar rats, with litters standardized to nine female pups, which were divided into two groups: normally nourished or overnourished. The groups were further subdivided into control and fluoxetine-treated groups, with the pharmacological treatment maintained until the 21st day of life. At 30 days of age, euthanasia was performed, and tissues from the hypothalamus and brainstem were collected.Results We observed an increase in body weight and the Lee index in the overnourished group, but fluoxetine treatment reduced these indices. Additionally, overnourished rats consumed more palatable food. Biochemically, NADH content in the hypothalamus was altered by overnutrition but restored by fluoxetine treatment. Citrate synthase activity was reduced in the overnourished group in the hypothalamus but increased in the brainstem of fluoxetine-treated rats. The production of reactive oxygen species was higher in the overnourished group, and oxidative stress biomarkers showed increased levels of MDA and protein carbonylation in these rats. Overnutrition impaired the antioxidant activity of enzymes in both the hypothalamus and brainstem, whereas fluoxetine treatment improved this activity. BDNF expression was higher in the fluoxetine-treated groups compared to the overnourished group.Discussion These results demonstrate the detrimental effects of maternal overnutrition on the development of female offspring and the therapeutic potential of serotonergic manipulation to mitigate the early effects of obesity, with tissue-specific variations.
dc.description.sponsorshipPrincess Nourah bint Abdulrahman University Researchers Supporting Project [PNURSP 2024R309]; Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia
dc.description.sponsorshipThe authors would like to thank Prince Sultan University for its support.
dc.identifier.doi10.3389/fmolb.2025.1564061
dc.identifier.issn2296-889X
dc.identifier.pmid40452918
dc.identifier.scopus2-s2.0-105007032940
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3389/fmolb.2025.1564061
dc.identifier.urihttps://hdl.handle.net/11616/108703
dc.identifier.volume12
dc.identifier.wosWOS:001499278800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers in Molecular Biosciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectobesity
dc.subjectserotonin
dc.subjectfluoxetine
dc.subjecthypothalamus
dc.subjectovernutrition
dc.subjectoxidative stress
dc.titleSerotoninergic modulation in the brainstem and hypothalamus of female overnourished rats: impact on mitochondrial markers, oxidative stress and BDNF mRNA levels
dc.typeArticle

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