Design of a lateral flow assay targeting the conserved NIID_2019-nCoV_N gene region for molecular viral diagnosis

Basit Öğe Kaydı
dc.contributor.authorDerin, D. Cam
dc.contributor.authorGultekin, E.
dc.date.accessioned2026-04-04T13:32:59Z
dc.date.available2026-04-04T13:32:59Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractSARS-CoV-2, the causative agent of the COVID-19 pandemic, remains a significant threat to global public health. Therefore, rapid and accurate detection of the virus continues to be of critical importance. Among the specific gene regions of SARS-CoV2, the Nucleocapsid (N) protein gene is one of the most frequently targeted for viral identification, with NIID_2019-nCOV_N being a notable example. While reverse transcriptase polymerase chain reaction (RT-PCR) remains the gold standard for diagnosis, alternative molecular detection methods are still limited. In this study, a lateral flow assay (LFA) was developed for the detection of a conserved gene region within NIID_2019-nCOV_N. Gold nanoparticles (AuNPs) were employed to enable visual detection, and the assay was designed based on nucleic acid hybridization principles. Two different membrane types (M17 and M12), three oligonucleotide probe concentrations (2, 4, and 8 mM) conjugated to AuNPs, and the assay's limit of detection (LOD) were evaluated. The target sequence from NIID_2019-nCOV_N was successfully detected by the naked eye within 5-6 min. No significant differences in performance were observed between the two membrane types across all probe concentrations, and the LOD was determined to be 1 pM. Consequently, the nucleic acid-based lateral flow assay (NABLFA) designed in this study, which targets a specific conserved base sequence, demonstrated high potential for rapid and sensitive molecular detection of SARS-CoV-2. Furthermore, this approach may be adapted for the identification of emerging viral variants or future outbreaks.
dc.description.sponsorshipInonu University Scientific Research Project [TOA-2020-2238]
dc.description.sponsorshipThis work was supported by Inonu University Scientific Research Project (BAP; TOA-2020-2238).
dc.identifier.doi10.1590/1414-431X2025e14761
dc.identifier.issn0100-879X
dc.identifier.issn1414-431X
dc.identifier.orcid0000-0001-5617-4067
dc.identifier.orcid0000-0002-8545-1868
dc.identifier.pmid41092196
dc.identifier.scopus2-s2.0-105018892854
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1590/1414-431X2025e14761
dc.identifier.urihttps://hdl.handle.net/11616/108847
dc.identifier.volume58
dc.identifier.wosWOS:001597743000001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAssoc Bras Divulg Cientifica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectLateral flow assay
dc.subjectMolecular diagnosis
dc.subjectNIID_2019-nCOV_N
dc.subjectSARS-CoV-2
dc.subjectRapid assay
dc.titleDesign of a lateral flow assay targeting the conserved NIID_2019-nCoV_N gene region for molecular viral diagnosis
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu