Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract on Hepatic Damage Generated by Alpha-Amanitine
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Tarih
2017
Dergi Başlığı
Dergi ISSN
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Abstract: Dünyada mantar zehirlenmelerine bağlı ölümlerin yaklaşık 90%'ına Amanita phalloides türü mantarlar neden olmaktadır. Alfa-amanitin, bisiklik oktapeptid yapıda bu zehirlenmelerden sorumlu ana yapıdır. Bu çalışmada, enginar sulu yaprak ekstresinin oluşturulan toksisite üzerine etkilerinin araştırılması amaçlanmıştır. Çalışmada, Sprague-Dawley cinsi 28 erkek rat rastgele 4 gruba ayrıldı. Gruplar, kontrol grubu (serum fizyolojik 0.1 mL ip), alfa-amanitin (3 mg/kg tek doz ip), enginar yaprak ekstresi (oral 1.5 g/kg, 14 gün) ve tedavi uygulanan (alfaamanitin 3 mg/kg tek doz ip+ enginar yaprak ekstresi oral 1.5 g/kg, 14 gün) olmak üzere belirlendi. Alfa-amanitinin karaciğer malondialdehit (MDA) düzeyini arttırdığı, süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px), katalaz (CAT) aktiviteleri ile glutatyon (GSH) düzeyini düşürdüğü tespit edilmiştir. Enginar sulu yaprak ekstresi takviyesinin MDA seviyesini düşürürken antioksidan parametrelerde ve histopatolojik bulgularda iyileşmeye neden olduğu saptanmıştır. Çalışma sonucunda, alfa-amanitin kaynaklı karaciğer hasarına karşı enginarın sulu ekstresinin kullanımının, tedaviye destek olarak kullanılabileceği düşünülmektedir.
Abstract: Approximately 90% of mushroom poisoning deaths in the world is caused by types of Amanita phalloides. Alpha-amanitine has a bicyclic octapeptide structure, which is the main structure responsible for these poisoning cases. In the present study, it was aimed to investigate effects of leaf extracts with artichoke extract on this toxicity. In the study, 28 male rats of Sprague-Dawley species were randomized to 4 groups. The groups were designed as control; receiving serum physiological solution of 0.1 mL intraperitoneally (ip), alpha-amanitine; receiving 3 mg/kg single dose ip, artichoke leaf extract; receiving 1.5 g/kg orally for 14 d, and treatment group¸ receiving alpha-amanitine 3 mg/kg single dose ip+artichoke leaf extract 1.5 g/kg orally for 14 d. It was determined that alpha-amanitine increased hepatic malondialdehyde (MDA) levels, and decrease superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities with decreasing glutathione (GSH) levels. The supplementation with extract with artichoke, decreased MDA levels, it improved antioxidant parameters, and histopathological findings, so it was decided that extract with artichoke juice might be beneficial in alpha-amanitine related hepatic damage.
Abstract: Approximately 90% of mushroom poisoning deaths in the world is caused by types of Amanita phalloides. Alpha-amanitine has a bicyclic octapeptide structure, which is the main structure responsible for these poisoning cases. In the present study, it was aimed to investigate effects of leaf extracts with artichoke extract on this toxicity. In the study, 28 male rats of Sprague-Dawley species were randomized to 4 groups. The groups were designed as control; receiving serum physiological solution of 0.1 mL intraperitoneally (ip), alpha-amanitine; receiving 3 mg/kg single dose ip, artichoke leaf extract; receiving 1.5 g/kg orally for 14 d, and treatment group¸ receiving alpha-amanitine 3 mg/kg single dose ip+artichoke leaf extract 1.5 g/kg orally for 14 d. It was determined that alpha-amanitine increased hepatic malondialdehyde (MDA) levels, and decrease superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities with decreasing glutathione (GSH) levels. The supplementation with extract with artichoke, decreased MDA levels, it improved antioxidant parameters, and histopathological findings, so it was decided that extract with artichoke juice might be beneficial in alpha-amanitine related hepatic damage.
Açıklama
Anahtar Kelimeler
Kaynak
Kafkas Üniversitesi Veteriner Fakültesi Dergisi
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
23
Sayı
1
Künye
KAYMAZ M. B,KANDEMİR F. M,PAMUKÇU E,ERÖKSÜZ Y,ÖZDEMİR N (2017). Effects of Aqueous Artichoke (Cynara scolymus) Leaf Extract
on Hepatic Damage Generated by Alpha-Amanitine. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(1), 155 - 160.
