Arabidopsis thaliana Ogg1 protein excises 8-hydroxyguanine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine from oxidatively damaged DNA containing multiple lesions

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dc.authoridRoldan-Arjona, Teresa/0000-0003-2842-3956
dc.authoridMorales-Ruiz, Teresa/0000-0002-6357-6977
dc.authoridJaruga, Pawel/0000-0001-9192-6084
dc.authorwosidJaruga, Pawel/M-4378-2015
dc.authorwosidRoldan-Arjona, Teresa/H-5976-2015
dc.authorwosidMorales-Ruiz, Teresa/L-4842-2014
dc.contributor.authorMorales-Ruiz, T
dc.contributor.authorBirincioglu, M
dc.contributor.authorJaruga, P
dc.contributor.authorRodriguez, H
dc.contributor.authorRoldan-Arjona, T
dc.contributor.authorDizdaroglu, M
dc.date.accessioned2024-08-04T20:13:36Z
dc.date.available2024-08-04T20:13:36Z
dc.date.issued2003
dc.departmentİnönü Üniversitesien_US
dc.description.abstractA functional homologue of eukaryotic Ogg1 proteins in the model plant Arabidopsis thaliana has recently been cloned, isolated, and characterized [Garcia-Ortiz, M. V., Ariza, R. R., and RoldanA Arjona, T. (2001) Plant Mol. Biol. 47,795-804]. This enzyme (AtOgg1) exhibits a high degree of sequence similarity in several highly conserved regions with Saccharomyces cerevisiae, Drosophila melanogaster, and human Ogg1 proteins. We investigated the substrate specificity and kinetics of AtOgg1 for excision of modified bases from oxidatively damaged DNA that contained multiple pyrimidine- and purine-derived lesions. Two different DNA substrates prepared by exposure to ionizing radiation in aqueous solution under N2O or air were used for this purpose. Gas chromatography/isotope-dilution mass spectrometry was applied to identify and quantify modified bases in DNA samples. Of the 17 modified bases identified in DNA samples, only 8-hydroxyguanine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine were significantly excised from both DNA substrates. This is in agreement with the substrate specificities of other eukaryotic Ogg1 proteins that had previously been studied under identical conditions. Excision depended on incubation time, enzyme concentration, and substrate concentration and followed Michaelis-Menten kinetics. A significant dependence of excision on the nature of DNA substrate was observed in accord with previous studies on other DNA glycosylases. A comparison of excision kinetics pointed to significant differences between AtOgg1 and other Ogg1 proteins. We also investigated the effect of base-pairing on the excision using double-stranded oligodeoxynucleotides that contained 8-OH-Gua paired with each of the four DNA bases. The activity of AtOgg1 was most effective on the 8-OH-Gua:C pair with some or very low activity on other pairs in agreement with the activity of other Ogg1 proteins. The results unequivocally show that AtOgg1 possesses common substrates with other eukaryotic Ogg1 proteins albeit significant differences between their excision kinetics.en_US
dc.identifier.doi10.1021/bi027226u
dc.identifier.endpage3095en_US
dc.identifier.issn0006-2960
dc.identifier.issue10en_US
dc.identifier.pmid12627976en_US
dc.identifier.scopus2-s2.0-0346034951en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage3089en_US
dc.identifier.urihttps://doi.org/10.1021/bi027226u
dc.identifier.urihttps://hdl.handle.net/11616/93701
dc.identifier.volume42en_US
dc.identifier.wosWOS:000181535300037en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Chemical Socen_US
dc.relation.ispartofBiochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChromatography Mass-Spectrometryen_US
dc.subjectXrs5 Nuclear Extractsen_US
dc.subjectEscherichia-Coli Nthen_US
dc.subjectSaccharomyces-Cerevisiaeen_US
dc.subjectSubstrate-Specificityen_US
dc.subjectIonizing-Radiationen_US
dc.subjectFpg Proteinen_US
dc.subjectGlycosylase Activityen_US
dc.subjectMolecular-Cloningen_US
dc.subjectClustered Damageen_US
dc.titleArabidopsis thaliana Ogg1 protein excises 8-hydroxyguanine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine from oxidatively damaged DNA containing multiple lesionsen_US
dc.typeArticleen_US

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