HSP70 immune reactivity and TUNEL positivity in the liver of toluene-inhaled and melatonin-treated rats

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dc.authoridOgeturk, Murat/0000-0002-5744-4812
dc.authoridkuloğlu, tuncay/0000-0001-9874-3838
dc.authoridKOCAMAN, NEVIN/0000-0002-6682-6345
dc.authorwosidOgeturk, Murat/B-3444-2008
dc.authorwosidkuloğlu, tuncay/W-2310-2018
dc.authorwosidKOCAMAN, NEVIN/V-9730-2018
dc.contributor.authorTas, Ufuk
dc.contributor.authorOgeturk, Murat
dc.contributor.authorKuloglu, Tuncay
dc.contributor.authorSapmaz, Hilal Irmak
dc.contributor.authorKocaman, Nevin
dc.contributor.authorZararsiz, Ismail
dc.contributor.authorSarsilmaz, Mustafa
dc.date.accessioned2024-08-04T20:37:42Z
dc.date.available2024-08-04T20:37:42Z
dc.date.issued2013
dc.departmentİnönü Üniversitesien_US
dc.description.abstractToluene is a clear, colorless and volatile hydrocarbon that is metabolized in liver, produced free oxygen radicals and can mediate cellular damage. Melatonin which is a pineal gland hormone is a very potent antioxidant. It can make the cellular membrane more durable against oxidative attacks and protect nuclear DNA from oxidative damage. This study aimed to investigate heat shock protein (HSP)70 immune reactivity and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) positivity (apoptotic activity) in the liver of toluene-inhaled and melatonin-treated rats. A total of 21 adult male Wistar albino rats were divided at random into 3 equal groups. Animals in group I were designated as control. The rats in group II were exposed to toluene (3000ppm/1h/day) for 30 days, while the rats in group III were treated with melatonin (10mg/kg/day, intraperitoneally) plus toluene inhalation. At the end of the 30-day experimental period, all rats were killed by decapitation. Then the liver tissues of rats were removed and tissue specimens were embedded in paraffin blocks. The specimens were stained with periodic acid-schiff (PAS) following routine histological procedures. Sections obtained from paraffin blocks were used for immune detection of TUNEL and HSP70. In light microscopic observations of tissues from toluene-inhaled rats, massive hepatocyte degeneration, ballooning degeneration and decreased PAS positivity were observed. Increased TUNEL positivity and HSP70 immune reactivity were determined in toluene-inhaled group and melatonin treatment decreased all these adverse effects.en_US
dc.identifier.doi10.1177/0748233712440138
dc.identifier.endpage522en_US
dc.identifier.issn0748-2337
dc.identifier.issn1477-0393
dc.identifier.issue6en_US
dc.identifier.pmid22438294en_US
dc.identifier.scopus2-s2.0-84879466227en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage514en_US
dc.identifier.urihttps://doi.org/10.1177/0748233712440138
dc.identifier.urihttps://hdl.handle.net/11616/96110
dc.identifier.volume29en_US
dc.identifier.wosWOS:000320703600005en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSage Publications Incen_US
dc.relation.ispartofToxicology and Industrial Healthen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTolueneen_US
dc.subjectmelatoninen_US
dc.subjectTUNELen_US
dc.subjectHSP70en_US
dc.subjectliveren_US
dc.titleHSP70 immune reactivity and TUNEL positivity in the liver of toluene-inhaled and melatonin-treated ratsen_US
dc.typeArticleen_US

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