Development and validation of an enzyme-linked immunosorbent assay for detection of cortisol in human saliva

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dc.authorscopusid56641616300
dc.authorscopusid7006165283
dc.authorscopusid57191495322
dc.contributor.authorOzgocer T.
dc.contributor.authorYildiz S.
dc.contributor.authorUçar C.
dc.date.accessioned2024-08-04T20:02:27Z
dc.date.available2024-08-04T20:02:27Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractNon-invasive measurement of cortisol in saliva is of prime importance as it represents a bioavailable neuroendocrine marker for stress. Therefore, in this study, we developed an enzyme immune assay that was suitable for salivary cortisol measurements. For that purpose, rabbit polyclonal antibody was raised against cortisol-3-CMO:BSA conjugate. The test was based on competition of liquid phase cortisol with conjugated cortisol on the solid phase. Primary antibody was used to bind available sites on the conjugate, which was proportional to numbers of cortisol in liquid phase. Biotinylated secondary anti-rabbit antibody was used to detect primary antibodies by addition of streptavidin peroxidase and substrate, respectively. Color formation was stopped and yellow color was read by a plate-reader spectrophotometer. Additionally, validated test was used to met all validation criteria including. Test developed was used to establish cortisol awakening response (CAR) in saliva samples collected in the morning after awakening (0, 15, 30, and 60th min) from women (n = 4) and men (n = 4) at 8 or 4 different days, respectively. Diurnal cortisol levels were assessed (n = 8) at after awaking 60 min at morning, 12:00, 19:00, and 22:00 hr. In conclusion, an enzyme immunoassay test was successfully produced, validated and used for cortisol measurement in saliva samples. © 2017 Taylor & Francis.en_US
dc.identifier.doi10.1080/15321819.2016.1230130
dc.identifier.endpage164en_US
dc.identifier.issn1532-1819
dc.identifier.issue2en_US
dc.identifier.pmid27600799en_US
dc.identifier.scopus2-s2.0-84991029185en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage147en_US
dc.identifier.urihttps://doi.org/10.1080/15321819.2016.1230130
dc.identifier.urihttps://hdl.handle.net/11616/91707
dc.identifier.volume38en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor and Francis Inc.en_US
dc.relation.ispartofJournal of Immunoassay and Immunochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcortisolen_US
dc.subjectenzyme immunoassaysen_US
dc.subjectnon-invasiveen_US
dc.subjectsalivaen_US
dc.titleDevelopment and validation of an enzyme-linked immunosorbent assay for detection of cortisol in human salivaen_US
dc.typeArticleen_US

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