Prevalence of rotavirus genotypes in children younger than 5 years of age before the introduction of a universal rotavirus vaccination program: Report of Rotavirus Surveillance in Turkey

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dc.authorscopusid57195695569
dc.authorscopusid24724572300
dc.authorscopusid56343258200
dc.authorscopusid35408618400
dc.authorscopusid54382004800
dc.authorscopusid8953204500
dc.authorscopusid6602497533
dc.contributor.authorDurmaz R.
dc.contributor.authorKalaycioglu A.T.
dc.contributor.authorAcar S.
dc.contributor.authorBakkaloglu Z.
dc.contributor.authorKaragoz A.
dc.contributor.authorKorukluoglu G.
dc.contributor.authorErtek M.
dc.date.accessioned2024-08-04T20:02:38Z
dc.date.available2024-08-04T20:02:38Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground: Group A rotaviruses are the most common causative agent of acute gastroenteritis among children less than 5 years of age throughout the world. This sentinel surveillance study was aimed to obtain baseline data on the rotavirus G and P genotypes across Turkey before the introduction of a universal rotavirus vaccination program. Methods: Rotavirus antigen-positive samples were collected from 2102 children less than 5 years of age who attended hospitals participating in the Turkish Rotavirus Surveillance Network. Rotavirus antigen was detected in the laboratories of participating hospitals by commercial serological tests such as latex agglutination, immunochromatographic test or enzyme immunoassay. Rotavirus G and P genotypes were determined by reverse transcription polymerase chain reaction (RT-PCR) using consensus primers detecting the VP7 and VP4 genes, followed by semi-nested type-specific multiplex PCR. Results: RT-PCR found rotavirus RNA in 1644 (78.2%) of the samples tested. The highest rate of rotavirus positivity (38.7%) was observed among children in the 13 to 24 month age group, followed by children in the age group of 25 to 36 months (28.3%). A total of eight different G types, six different P types, and 42 different G-P combinations were obtained. Four common G types (G1, G2, G3, and G9) and two common P types (P[8] and P[4]) accounted for 95.1% and 98.8% of the strains, respectively. G9P[8] was the most common G/P combination found in 40.5% of the strains followed by G1P[8] (21.6%), G2P[8] (9.3%), G2P[4] (6.5%), G3P[8] (3.5%), and finally, G4P[8] (3.4%). These six common genotypes included 83.7% of the strains tested in this study. The rate of uncommon genotypes was 14%. Conclusion: The majority of the strains analyzed belonged to the G1-G4 and G9 genotypes, suggesting high coverage of current rotavirus vaccines. This study also demonstrates a dramatic increase in G9 genotype across the country. © 2014 Durmaz et al.en_US
dc.identifier.doi10.1371/journal.pone.0113674
dc.identifier.issn1932-6203
dc.identifier.issue12en_US
dc.identifier.pmid25437502en_US
dc.identifier.scopus2-s2.0-84914690138en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0113674
dc.identifier.urihttps://hdl.handle.net/11616/91840
dc.identifier.volume9en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS ONEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectmembrane proteinen_US
dc.subjectRotavirus antigenen_US
dc.subjectRotavirus vaccineen_US
dc.subjectunclassified drugen_US
dc.subjectvirus antigenen_US
dc.subjectvirus RNAen_US
dc.subjectcapsid proteinen_US
dc.subjectRotavirus vaccineen_US
dc.subjectvirus antigenen_US
dc.subjectvirus RNAen_US
dc.subjectVP4 protein, Rotavirusen_US
dc.subjectVP7 protein, Rotavirusen_US
dc.subjectArticleen_US
dc.subjectchilden_US
dc.subjectconsensusen_US
dc.subjectdisease surveillanceen_US
dc.subjectenzyme immunoassayen_US
dc.subjectgeneen_US
dc.subjectgene sequenceen_US
dc.subjectgenetic analysisen_US
dc.subjectgenotypeen_US
dc.subjecthumanen_US
dc.subjectimmunoaffinity chromatographyen_US
dc.subjectlatex agglutination testen_US
dc.subjectmultiplex polymerase chain reactionen_US
dc.subjectpreschool childen_US
dc.subjectprevalenceen_US
dc.subjectreverse transcription polymerase chain reactionen_US
dc.subjectRotavirusen_US
dc.subjectRotavirus Gen_US
dc.subjectRotavirus infectionen_US
dc.subjectRotavirus Pen_US
dc.subjectserologyen_US
dc.subjectTurkey (republic)en_US
dc.subjectvaccinationen_US
dc.subjectvirus strainen_US
dc.subjectVP4 geneen_US
dc.subjectVP7 geneen_US
dc.subjectgeneticsen_US
dc.subjecthealth surveyen_US
dc.subjectimmunologyen_US
dc.subjectinfanten_US
dc.subjectRotavirusen_US
dc.subjectRotavirus Infectionsen_US
dc.subjectsequence analysisen_US
dc.subjectTurkeyen_US
dc.subjectvirologyen_US
dc.subjectRotavirusen_US
dc.subjectAntigens, Viralen_US
dc.subjectCapsid Proteinsen_US
dc.subjectChild, Preschoolen_US
dc.subjectGenotypeen_US
dc.subjectHumansen_US
dc.subjectInfanten_US
dc.subjectPrevalenceen_US
dc.subjectPublic Health Surveillanceen_US
dc.subjectRNA, Viralen_US
dc.subjectRotavirusen_US
dc.subjectRotavirus Infectionsen_US
dc.subjectRotavirus Vaccinesen_US
dc.subjectSequence Analysis, RNAen_US
dc.subjectTurkeyen_US
dc.titlePrevalence of rotavirus genotypes in children younger than 5 years of age before the introduction of a universal rotavirus vaccination program: Report of Rotavirus Surveillance in Turkeyen_US
dc.typeArticleen_US

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