Rhamnolipid production by Pseudomonas aeruginosa engineered with the Vitreoscilla hemoglobin gene

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dc.authoridKahraman, Hüseyin/0000-0001-6235-5497
dc.authorwosidKahraman, Hüseyin/ABG-9650-2020
dc.contributor.authorKahraman, H.
dc.contributor.authorErenler, S. O.
dc.date.accessioned2024-08-04T20:35:46Z
dc.date.available2024-08-04T20:35:46Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe potential of Pseudomonas aeruginosa expressing the Vitreoscilla hemoglobin gene (vgb) for rhamnolipid production was studied. P. aeruginosa (NRRL B-771) and its transposon mediated vgb transferred recombinant strain, PaJC, were used in the research. The optimization of rhamnolipid production was carried out in the different conditions of cultivation (agitation rate, the composition of culture medium and temperature) in a time-course manner. The nutrient source, especially the carbon type, had a dramatic effect on rhamnolipid production. The PaJC strain and the wild type cells of P. aeruginosa started producing biosurfactant at the stationary phase and its concentration reached maximum at 24 h (838 mg/l(-1)) and at 72 h (751 mg l(-1)) of the incubation respectively. Rhamnolipid production was optimal in batch cultures when the temperature and agitation rate were controlled at 30A degrees C and 100 rpm. It reached 8373 mg l(-1) when the PaJC cells were grown in 1.0% glucose supplemented minimal media. Genetic engineering of biosurfactant producing strains with vgb may be an effective method to increase its production.en_US
dc.identifier.doi10.1134/S000368381202007X
dc.identifier.endpage193en_US
dc.identifier.issn0003-6838
dc.identifier.issn1608-3024
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84857661341en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage188en_US
dc.identifier.urihttps://doi.org/10.1134/S000368381202007X
dc.identifier.urihttps://hdl.handle.net/11616/95584
dc.identifier.volume48en_US
dc.identifier.wosWOS:000300886400009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPleiades Publishing Incen_US
dc.relation.ispartofApplied Biochemistry and Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiosurfactantsen_US
dc.subjectExpressionen_US
dc.titleRhamnolipid production by Pseudomonas aeruginosa engineered with the Vitreoscilla hemoglobin geneen_US
dc.typeArticleen_US

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