Detection of Hypervirulent ST-17 Clone Among Clinical Group B Streptococcus Isolates Using MALDI-TOF MS and PCR

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dc.contributor.authorMalkocoglu, Gulsah
dc.contributor.authorBulut, Mehmet Emin
dc.contributor.authorBayraktar, Banu
dc.contributor.authorOtlu, Baris
dc.contributor.authorAktas, Elif
dc.date.accessioned2026-04-04T13:30:54Z
dc.date.available2026-04-04T13:30:54Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractObjective: Group B streptococcus (GBS) is the leading causative agent of neonatal morbidity and mortality. Sequence type 17 (ST-17) in GBS causes neonatal invasive disease more frequently than other STs. This study aimed to investigate the presence of hypervirulent ST-17 in a collection of clinical GBS isolates. Materials and Methods: GBS isolates obtained from patients with invasive and non-invasive infections were included in the study. For the detection of ST-17 GBS, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and polymerase chain reaction (PCR) methods were performed. Multilocus sequence typing (MLST) was also performed in a subset of some representative GBS strains. Results: Among 108 GBS isolates included in the study, 6 (5.5%) were identified as ST-17 by MALDI-TOF MS. Discriminatory peaks were detected at 7620 Da for ST-17 and 7638 Da for non-ST-17 isolates. In addition to six isolates that were positive for ST-17 by MALDI-TOF MS, one more isolate (GBS2) was found to be positive for ST-17 by PCR test. MLST revealed that those six isolates were ST-17 or single-locus variants of ST-17, while the isolate GBS2 was ST-1. Among the remaining 20 representative GBS isolates, 14 STs were identified by MLST, and all of them were non-ST-17 in accordance with MALDI-TOF MS and PCR results. Conclusion: In this study, the presence of a circulating hypervirulent ST-17 clone in T & uuml;rkiye was demonstrated for the first time. MALDI-TOF MS successfully and rapidly detected ST-17 and non-ST-17 GBS isolates. This practical method may contribute to efficiently managing neonatal infections caused by ST-17 GBS.
dc.identifier.doi10.36519/idcm.2025.455
dc.identifier.endpage173
dc.identifier.issn2667-646X
dc.identifier.issue2
dc.identifier.orcid0000-0003-2097-3224
dc.identifier.orcid0000-0002-6220-0521
dc.identifier.pmid40657014
dc.identifier.scopus2-s2.0-105009418800
dc.identifier.scopusqualityN/A
dc.identifier.startpage166
dc.identifier.urihttps://doi.org/10.36519/idcm.2025.455
dc.identifier.urihttps://hdl.handle.net/11616/108461
dc.identifier.volume7
dc.identifier.wosWOS:001522211100006
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherDoc Design Informatics Co Ltd
dc.relation.ispartofInfectious Diseases and Clinical Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectGroup B Streptococcus
dc.subjectST-17
dc.subjectMALDI-TOF MS
dc.subjectPCR
dc.subjectMLST
dc.titleDetection of Hypervirulent ST-17 Clone Among Clinical Group B Streptococcus Isolates Using MALDI-TOF MS and PCR
dc.typeArticle

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