Effects of larazotide acetate, a tight junction regulator, on the liver and intestinal damage in acute liver failure in rats

Basit Öğe Kaydı
dc.authoridSAĞLAM, Osman/0000-0003-0779-992X
dc.authoridUremis, Muhammed Mehdi/0000-0003-2296-2422
dc.authoridHarputluoglu, Muhsin Murat Muhip/0000-0002-9415-147X
dc.authoridYILMAZ, Ismet/0000-0001-8680-3098
dc.authoridUremis, Nuray/0000-0002-3958-4352
dc.authoridCALISKAN, ALI RIZA/0000-0003-3187-8548
dc.authoridTikici, Deniz/0000-0003-1759-2973
dc.authorwosidSAĞLAM, Osman/ABI-6265-2020
dc.authorwosidUremis, Muhammed Mehdi/HKP-0531-2023
dc.authorwosidHarputluoglu, Muhsin Murat Muhip/ABI-3094-2020
dc.contributor.authorCaliskan, Ali Riza
dc.contributor.authorGul, Mehmet
dc.contributor.authorYilmaz, Ismet
dc.contributor.authorOtlu, Baris
dc.contributor.authorUremis, Nuray
dc.contributor.authorUremis, Muhammed Mehdi
dc.contributor.authorKilicaslan, Ilkay
dc.date.accessioned2024-08-04T20:50:52Z
dc.date.available2024-08-04T20:50:52Z
dc.date.issued2021
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground and Aim The epithelial cells are the strongest determinants of the physical intestinal barrier. Tight junctions (TJs) hold the epithelial cells together and allow for selective paracellular permeability. Larazotide acetate (LA) is a synthetic octapeptide that reduces TJ permeability by blocking zonulin receptors. In this study, we aimed to investigate the effects of LA, a TJ regulator, on the liver and intestinal histology in the model of acute liver failure (ALF) in rats. Materials and Methods The thioacetamide (TAA) group received intraperitoneal (ip) injections of 300 mg/kg TAA for 3 days. The TAA+LA(dw) (drinking water) group received prophylactic 0.01 mg/mL LA orally for 7 days before the first dose of TAA. The LA(dw) group received 0.01 mg/mL LA orally. The TAA + LA(g) (gavage) group received prophylactic 0.01 mg/mL LA via oral gavage for 7 days before the first dose of TAA. The LA(g) group received 0.01 mg/mL LA via oral gavage. While liver tissue was evaluated only with light microscopy, intestinal samples were examined with light and electron microscopy. Results Serum ammonia, AST, and ALT levels in the TAA group were significantly higher than in control groups (all p < 0.01). Serum ALT levels in the TAA + LA(dw) group were significantly lower than in the TAA group (p < 0.05). However, serum ammonia and ALT levels did not differ between the TAA and other groups. Serious liver damage in the TAA group was accompanied by marked intestinal damage. There was no significant difference between the TAA and TAA + LA(dw) groups and TAA and TAA + LA(g) groups for liver damage scores. However, intestinal damage scores significantly decreased in the TAA + LA(dw) group compared to the TAA group. In the TAA + LA(dw) group, fusion occurred between the surface epithelial cells of neighboring villi and connecting regions formed as epithelial bridges between the villi. Conclusion Our findings suggest that LA reduced intestinal damage by acting on TJs in the TAA-induced ALF model in rats.en_US
dc.description.sponsorshipInonu University Scientific Research Projects Unit [TSA-2019-1601]en_US
dc.description.sponsorshipThe author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by the Inonu University Scientific Research Projects Unit (Project number: TSA-2019-1601).en_US
dc.identifier.doi10.1177/09603271211058882
dc.identifier.endpageS701en_US
dc.identifier.issn0960-3271
dc.identifier.issn1477-0903
dc.identifier.issue12_SUPPLen_US
dc.identifier.pmid34791921en_US
dc.identifier.scopus2-s2.0-85119523911en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpageS693en_US
dc.identifier.urihttps://doi.org/10.1177/09603271211058882
dc.identifier.urihttps://hdl.handle.net/11616/100320
dc.identifier.volume40en_US
dc.identifier.wosWOS:000721352100001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSage Publications Ltden_US
dc.relation.ispartofHuman & Experimental Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLarazotide acetateen_US
dc.subjectceliac diseaseen_US
dc.subjectliver failureen_US
dc.subjecttight junctionen_US
dc.titleEffects of larazotide acetate, a tight junction regulator, on the liver and intestinal damage in acute liver failure in ratsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu