Simultaneous Determination of Water and Fat-Soluble Vitamins in Pekmez Samples by High Performance Liquid Chromatography Coupled with Diode Array Detection
Küçük Resim Yok
Tarih
2010
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Asian Journal Of Chemistry
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In the present work, a RP-HPLC procedure has been applied for the determination of water-soluble vitamins (riboflavin. nicotinamide. pantothenic acid. pyridoxine hydrochloride. pyridoxal. folic acid, ascorbic acid) and tat-soluble vitamins (trans-retinol. alpha-tocopherol) in pekmez samples. a tradional Turkish concentrate fruit food The sample treatment proposed consists of a solid-phase extraction with Sep-Pak C(18) cartridges that allow the seperation of fat-soluble vitamins, which were retained on the sorbent, from water-soluble vitamins. After isolation of the two fractions, the water-soluble vitamins were analyzed by HPLC on an ACE 5 C(18) (250 x 4.6 mm, 5 mu m) analytical column using 0.1 mol L(-1) KH(2)PO(4) (pH 7 0)-CH(3)OH as mobile phase The chromatographic analysis of the fat-soluble vitamins was earned out after their sequential elution with CH(3)OH-CCl(4) from C(18) sorbent. on the above column. Afterwards. the fat-soluble vitamins were analyzed on an ACE 5 C(18) analytical with isocratic mobile phase consisting of CH(3)OH-CH(3)CN at a flow-rate of 2 mL min(-1). Identification of compounds was achieved by comparing their retention times and UV spectra with those of standards stored in a data bank The accuracy of the method was tested obtaining an average recovery ranging between 91 and 110%.
Açıklama
Anahtar Kelimeler
Water-soluble vitamins, Fat-soluble vitamins, HPLC, Pekmez
Kaynak
Asian Journal of Chemistry
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
22
Sayı
5
