Radical scavenging activity and vitexin content of Vitex agnus castus leaves and fruits
| dc.authorid | TR21083 | en_US |
| dc.authorid | TR184256 | en_US |
| dc.authorid | TR100937 | en_US |
| dc.authorid | TR4249 | en_US |
| dc.contributor.author | Gökbulut, Alper | |
| dc.contributor.author | Özhan, Onural | |
| dc.contributor.author | Karacaoğlu, Melek | |
| dc.contributor.author | Şarer, Engin | |
| dc.date.accessioned | 2017-04-18T11:02:27Z | |
| dc.date.available | 2017-04-18T11:02:27Z | |
| dc.date.issued | 2012 | |
| dc.department | İnönü Üniversitesi | en_US |
| dc.description.abstract | The goal of this study was to determine some of the important phenolic constituents of the methanolic extracts of the leaves and fruits of Vitex agnus-castus by RP-HPLC with regard to the results obtained from radical scavenging activity and total phenolic content assays. 1,1-diphenyl-2-picrylhydrazyl (DPPH) test was used to assess the radical scavenging potential of the extracts. Both leaf and fruit extracts exhibited significant radical scavenging activity with IC50 values of 0.449±0.001 mg/mL and 0.612±0.004 mg/mL, respectively. By using Folin-Ciocalteu’s method, the total phenolic content of leaves and fruits of the plant was determined as 123.9±2.281 mg GAE/g extract and 114.5±2.704 mg GAE/g extract, respectively. In continuation of our ongoing studies on the phenolics of Vitex agnus-castus, a simple and accurate HPLC method was used to determine some phenolic compounds such as vitexin, ferulic acid, rutin, and luteolin qualitatively and quantitatively. Contrary to the previous literature, ferulic acid and rutin were determined neither in leaf nor in fruit extracts, while luteolin was determined in trace amount only in fruit extract. Vitexin, one of the important flavonoid constituents of Vitex agnus-castus, was determined in significant amount as 0.252±0.0089% and 0.342±0.0153% in the leaves and fruits, respectively. The results showed that vitexin is partially responsible for the antioxidant potential of the extracts due to its significant amount and the standardization of the extracts should be performed on vitexin by this suitable RP-HPLC method. | en_US |
| dc.description.abstract | Bu çalışmanın amacı, radikal süpürücü aktivite ve total fenol miktar tayini deneylerinden elde edilen sonuçlara dayanarak Vitex agnus-castus yaprak ve meyvalarının metanollü ekstrelerinin bazı önemli fenolik bileşenlerini TF-YBSK ile tayin etmektir. Ekstrelerin radikal süpürücü aktivite potansiyelleri 1,1-difenil-2-pikrilhidrazil (DPPH) testi kullanılarak değerlendirilmiştir. Sırasıyla, 0,449±0,001 mg/mL ve 0,612±0,004 mg/mL IC50 değerleriyle, yaprak ve meyva ekstrelerinin her ikisi de belirgin derecede radikal süpürücü etki göstermiştir. Bitkinin yaprak ve meyvalarının total fenol içeriği Folin Ciocalteu yöntemi kullanılarak, sırasıyla, 123,9±2,281 mg GAE/g ekstre ve 114,5±2,704 mg GAE/g ekstre olarak tayin edilmiştir. Vitex agnus-castus’un fenolik bileşikleri üzerindeki çalışmalarımızın devamı olarak, viteksin, ferulik asit, rutin ve luteolin gibi bazı fenolik bileşiklerin kalitatif ve kantitatif tayini için basit ve hassas bir YBSK yöntemi kullanılmıştır. Literatür verilerinin aksine, hem yaprak hem de meyva ekstrelerinde ferulik asit ve rutine rastlanmazken, luteolin sadece meyva ekstresinde eser miktarda saptanmıştır. Vitex agnus-castus’un önemli flavonoit bileşenlerinden biri olan viteksin, yaprak ve meyvalarda sırasıyla %0,252±0,0089 ve %0,342±0,0153 olarak belirgin miktarlarda saptanmıştır. Sonuçlar, viteksinin bitkideki yüksek miktarına bağlı olarak ekstrelerin antioksidan potansiyelinden kısmi olarak sorumlu olduğunu ve ekstrelerin standardizasyonunun viteksin üzerinden bu elverişli TFYBSK yöntemi ile gerçekleştirilebileceğini göstermektedir | en_US |
| dc.identifier.citation | Gökbulut, A. Özhan, O. Karacaoğlu, M. Şarer, E. (2012). Radical scavenging activity and vitexin content of Vitex agnus castus leaves and fruits. FABAD Journal of Pharmaceutical Sciences. 35, 85–91. | en_US |
| dc.identifier.endpage | 91 | en_US |
| dc.identifier.startpage | 85 | en_US |
| dc.identifier.uri | https://www.researchgate.net/profile/Alper_Goekbulut/publication/265790049_Radical_scavenging_activity_and_vitexin_content_of_Vitex_agnus-castus_leaves_and_fruits/links/5426d8410cf26120b7b34526.pdf | |
| dc.identifier.uri | https://hdl.handle.net/11616/6694 | |
| dc.identifier.volume | 35 | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | FABAD Journal of Pharmaceutical Sciences | en_US |
| dc.relation.ispartof | :FABAD Journal of Pharmaceutical Sciences | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Vitex agnus-castus | en_US |
| dc.subject | Antioxidant activity | en_US |
| dc.subject | Vitexin | en_US |
| dc.subject | DPPH | en_US |
| dc.subject | RP-HPLC | en_US |
| dc.subject | Total phenolic content | en_US |
| dc.subject | Antioksidan aktivite | en_US |
| dc.subject | Viteksin | en_US |
| dc.subject | TF-YBSK | en_US |
| dc.subject | Total fenol miktarı | en_US |
| dc.title | Radical scavenging activity and vitexin content of Vitex agnus castus leaves and fruits | en_US |
| dc.type | Article | en_US |
