Evaluation of the Carbapenem Inactivation Method for Detection of Carbapenemase-Producing Gram-Negative Bacteria in Comparison with the RAPIDEC CARBA NP

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dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authoridSANDALLI, Cemal/0000-0002-1298-3687
dc.authoridComert, Fusun/0000-0003-0161-6897
dc.authoridaktas, elif/0000-0003-3087-5425
dc.authoridCOPUR CICEK, AYSEGUL/0000-0002-3673-9670
dc.authorwosidSANDALLI, Cemal/ABG-1089-2021
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.authorwosidÇİÇEK, Ayşegül ÇOPUR/AAM-6146-2021
dc.authorwosidkulah, canan/M-7058-2019
dc.authorwosidaktas, elif/ACL-9792-2022
dc.authorwosidBulut, Emin/AAY-8628-2021
dc.contributor.authorAktas, Elif
dc.contributor.authorMalkocoglu, Gulsah
dc.contributor.authorOtlu, Baris
dc.contributor.authorCicek, Aysegul Copur
dc.contributor.authorKulah, Canan
dc.contributor.authorComert, Fusun
dc.contributor.authorSandalli, Cemal
dc.date.accessioned2024-08-04T20:43:11Z
dc.date.available2024-08-04T20:43:11Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractTimely detection of carbapenemases by both phenotypic and genotypic methods is essential for developing strategies to control the spread of infections by carbapenem-resistant isolates and related morbidity and mortality. The aim of this study was to compare the performance of a commercial kit, the RAPIDEC (R) CARBA NP, and an in-house technique, the carbapenem inactivation method (CIM), against a panel of 136 carbapenemase and noncarbapenemase-producing Enterobacteriaceae, Acinetobacter baumannii, and Pseudomonas aeruginosa isolates. RAPIDEC CARBA NP displayed 99% sensitivity and 100% specificity, whereas the sensitivity and specificity were 78% and 100% for the CIM test, respectively. A slight modification of the CIM test, a prolonged incubation time of 4 hours instead of two, increased the sensitivity of the test to 90% by diminishing false negativity particularly for A. baumannii. In conclusion, both tests possess a high performance and are practical for the detection of carbapenemases. Although RAPIDEC CARBA NP is a more rapid and reliable method, the CIM test may represent a useful tool for microbiology laboratories due to its simplicity and availability at any laboratory with low cost.en_US
dc.description.sponsorshipSisli Hamidiye Etfal Training and Research Hospitalen_US
dc.description.sponsorshipThis work was supported by internal funding of Sisli Hamidiye Etfal Training and Research Hospital (decision date: July 12, 2015).en_US
dc.identifier.doi10.1089/mdr.2016.0092
dc.identifier.endpage+en_US
dc.identifier.issn1076-6294
dc.identifier.issn1931-8448
dc.identifier.issue4en_US
dc.identifier.pmid27575912en_US
dc.identifier.scopus2-s2.0-85020193946en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage457en_US
dc.identifier.urihttps://doi.org/10.1089/mdr.2016.0092
dc.identifier.urihttps://hdl.handle.net/11616/97842
dc.identifier.volume23en_US
dc.identifier.wosWOS:000402567800007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofMicrobial Drug Resistanceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCIMen_US
dc.subjectCARBA NPen_US
dc.subjectcarbapenemaseen_US
dc.titleEvaluation of the Carbapenem Inactivation Method for Detection of Carbapenemase-Producing Gram-Negative Bacteria in Comparison with the RAPIDEC CARBA NPen_US
dc.typeArticleen_US

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