Adenovirus-mediated gene therapy in an experimental model of breast cancer metastatic to the brain

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dc.authorscopusid58021718900
dc.authorscopusid35433519900
dc.authorscopusid55733359900
dc.authorscopusid59113529500
dc.authorscopusid35380023700
dc.authorscopusid7005928213
dc.contributor.authorÇolak A.
dc.contributor.authorGoodman J.C.
dc.contributor.authorChen S.-H.
dc.contributor.authorWoo S.L.C.
dc.contributor.authorGrossman R.G.
dc.contributor.authorShine H.D.
dc.date.accessioned2024-08-04T20:00:44Z
dc.date.available2024-08-04T20:00:44Z
dc.date.issued1995
dc.departmentİnönü Üniversitesien_US
dc.description.abstractWe investigated the therapeutic efficacy of adenovirus-mediated gene therapy to treat malignant mammary tumors in vitro and in vivo in the brain. A mammary adenocarcinoma cell line derived from Fischer rats (13762 MAT B III; MAT-B) was used. In vitro studies demonstrated that the MAT-B cells could be efficiently transduced with a replication-defective adenovirus (ADV) vector that carried the herpes simplex virus gene for thymidine kinase (ADV-tk), and that ADV-tk transduction rendered the MAT-B cells sensitive to killing, in a dose-dependent manner, with ganciclovir (GCV). An animal model of a mammary tumor metastatic to the brain was produced by injecting MAT-B cells into the caudate nucleus of Fischer rats. Seven days after MAT-B cell injection, when the tumors were approximately 5 mm2 in cross-sectional size, the tumors were injected with ADV-tk or a control adenovirus vector containing the ?-galactosidase (?-Gal) gene (ADV-?gal). After vector injection the animals were treated with GCV or with saline for 6 days. Sixteen days after tumor cell injection, the brains were examined histologically. The rats that were injected with ADV-?gal and treated with GCV or saline, and those that were injected with ADV-tk and treated with saline had large tumors, whereas the rats that were injected with ADV-tk and treated with GCV had no visible tumor tissue at the site of tumor cell injection. In survival studies animals treated with ADV-tk + GCV survived a significantly longer time than animals treated with ADV-?gal + GCV. Our results demonstrate that the recombinant adenoviral vector containing the tk gene confers GCV cytotoxic sensitivity to mammary tumor cells in vitro and in the brain, and suggest that this treatment strategy may be useful in treating somatic tumors that metastasize to the brain.en_US
dc.identifier.endpage1322en_US
dc.identifier.issn1043-0342
dc.identifier.issue10en_US
dc.identifier.pmid8590736en_US
dc.identifier.scopus2-s2.0-0028881916en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1317en_US
dc.identifier.urihttps://hdl.handle.net/11616/90950
dc.identifier.volume6en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert Inc.en_US
dc.relation.ispartofHuman Gene Therapyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbeta galactosidaseen_US
dc.subjectgancicloviren_US
dc.subjectthymidine kinaseen_US
dc.subjectAdenovirusen_US
dc.subjectanimal cellen_US
dc.subjectanimal experimenten_US
dc.subjectanimal tissueen_US
dc.subjectarticleen_US
dc.subjectbrain metastasisen_US
dc.subjectbreast adenocarcinomaen_US
dc.subjectcancer cell cultureen_US
dc.subjectcancer modelen_US
dc.subjectcaudate nucleusen_US
dc.subjectcontrolled studyen_US
dc.subjectdrug cytotoxicityen_US
dc.subjectdrug sensitivityen_US
dc.subjectgene therapyen_US
dc.subjectgenetic transductionen_US
dc.subjectHerpes simplex virusen_US
dc.subjectnonhumanen_US
dc.subjectraten_US
dc.subjectsurvival rateen_US
dc.subjectvirus geneen_US
dc.subjectvirus recombinanten_US
dc.subjectAdenoviridaeen_US
dc.subjectAleutian mink disease parvovirus (STRAIN G)en_US
dc.subjectAnimaliaen_US
dc.subjectHerpesen_US
dc.subjectSimplexvirusen_US
dc.titleAdenovirus-mediated gene therapy in an experimental model of breast cancer metastatic to the brainen_US
dc.typeArticleen_US

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