Comparative assessment of in vitro and in vivo toxicity of azinphos methyl and its commercial formulation

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dc.authoridUÇKUN, Miraç/0000-0002-9018-8515
dc.authoridGüngördü, Abbas/0000-0001-7770-0660;
dc.authorwosidUÇKUN, Miraç/ABC-4209-2021
dc.authorwosidGüngördü, Abbas/A-3009-2015
dc.authorwosidMiraç, Uçkun/ABG-5824-2021
dc.contributor.authorGungordu, Abbas
dc.contributor.authorUckun, Mirac
dc.date.accessioned2024-08-04T20:42:44Z
dc.date.available2024-08-04T20:42:44Z
dc.date.issued2015
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe toxic effects of Gusathion (GUS), which is a commercial organophosphate (OP) pesticide, and also its active ingredient, azinphos methyl (AzM), are evaluated comparatively with in vitro and in vivo studies. Initially, the 96-h LC50 values of AzM and GUS were estimated for two different life stages of Xenopus laevis, embryos, and tadpoles. The actual AzM concentrations in exposure media were monitored by high-performance liquid chromatography. Also, the sub-lethal effects of these compounds to tadpoles were determined 24 h later at exposure concentrations of 0.1 and 1 mg/L using selected biomarker enzymes such as acetylcholinesterase (AChE), carboxylesterase (CaE), glutathione S-transferase (GST), glutathione reductase, lactate dehydrogenase, and aspartate aminotrasferase. Differences in AChE inhibition capacities of AzM and GUS were evaluated under in vitro conditions between frogs and fish in the second part of this study. The AChE activities in a pure electrical eel AChE solution and in brain homogenates of adult Cyprinus carpio, Pelophylax ridibundus, and X. laevis were assayed after in vitro exposure to 0.05, 0.5, 5, and 50 mg/L concentrations of AzM and GUS. According to in vivo studies AChE, CaE and GST are important biomarkers of the effect of OP exposure while CaE may be more effective in short-term, low-concentration exposures. The results of in vitro studies showed that amphibian brain AChEs were relatively more resistant to OP exposure than fish AChEs. The resistance may be the cause of the lower toxicity/lethality of OP compounds to amphibians than to fish. (c) 2014 Wiley Periodicals, Inc. Environ Toxicol 30: 1091-1101, 2015.en_US
dc.identifier.doi10.1002/tox.21982
dc.identifier.endpage1101en_US
dc.identifier.issn1520-4081
dc.identifier.issn1522-7278
dc.identifier.issue9en_US
dc.identifier.pmid24616035en_US
dc.identifier.scopus2-s2.0-84994172068en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1091en_US
dc.identifier.urihttps://doi.org/10.1002/tox.21982
dc.identifier.urihttps://hdl.handle.net/11616/97562
dc.identifier.volume30en_US
dc.identifier.wosWOS:000359859100010en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofEnvironmental Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectazinphos methylen_US
dc.subjectGusathionen_US
dc.subjectbiomarkeren_US
dc.subjectfishen_US
dc.subjectfrogen_US
dc.titleComparative assessment of in vitro and in vivo toxicity of azinphos methyl and its commercial formulationen_US
dc.typeArticleen_US

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